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Literature summary for 1.1.1.8 extracted from

  • Yamada, M.
    Studies on L-glycerol-3-phosphate dehydrogenases in Japanese quail, Coturnix coturnix japonica. I. Multiple components and some enzymatic properties of nicotinamide adenine dinucleotide-linked L-glycerol-3-phosphate dehydrogenase from liver and testes (1972), J. Biochem., 72, 1081-1086.
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
alpha-glycerophosphate Km values from five different isoelectric focused components Coturnix japonica
0.33
-
alpha-glycerophosphate testes Coturnix japonica
3.39
-
alpha-glycerophosphate liver Coturnix japonica

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Coturnix japonica
-
dihydroxyacetone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Coturnix japonica
-
japanese quail
-

Purification (Commentary)

Purification (Comment) Organism
4 isozymes in testes and 5 isozymes in liver, distinct in isoelectric focusing Coturnix japonica

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Coturnix japonica
-
testis
-
Coturnix japonica
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
7.1
-
standard deviation 0.2, crude preparation, testes Coturnix japonica
33
-
isozyme P-V, liver Coturnix japonica
70.7
-
standard deviation 5.1, crude preparation, liver Coturnix japonica
96
-
isozyme P-I, liver Coturnix japonica
123
-
isozyme P-IV, liver Coturnix japonica
253
-
isozyme P-II, liver Coturnix japonica
806
-
isozyme P-III, liver Coturnix japonica

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
sn-glycerol 3-phosphate + NAD+
-
Coturnix japonica dihydroxyacetone phosphate + NADH + H+
-
r

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
25
-
isozymes P-I, P-V, liver Coturnix japonica
35
-
isozymes P-II, P-III, P-IV, liver Coturnix japonica

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
additional information
-
optimum pH values for components P-I, P-II, PIV, P-V range between pH 8.2 and 8.6, liver Coturnix japonica
9.3
-
liver, component P-III, pH optimum of testis enzyme also around pH 9.3 Coturnix japonica

pH Range

pH Minimum pH Maximum Comment Organism
8 10 about half-maximal activity at pH 8.0 and 10.0, oxidation of glycerol 3-phosphate, isozymes P-III, liver Coturnix japonica

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Coturnix japonica