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Literature summary for 1.1.1.8 extracted from

  • Baranowski, T.
    alpha-Glycerophosphate dehydrogenase (1963), The Enzymes, 2nd Ed. (Boyer, P. D. , Lardy, H. , Myrbńck, K. , eds. ), 7, 85-96.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
EDTA without EDTA the rates are about 60% of the maximal rate Oryctolagus cuniculus
glycine buffer 0.9 M, slight activation Oryctolagus cuniculus

Crystallization (Commentary)

Crystallization (Comment) Organism
-
Oryctolagus cuniculus

General Stability

General Stability Organism
charcoal inactivates rabbit enzyme, restorable by thiamic acid Oryctolagus cuniculus
EDTA stabilizes Oryctolagus cuniculus
even cold dilute solutions in 0.2 M ammonium sulfate at pH 5.8 are stable for weeks Oryctolagus cuniculus
redistilled water prevents denaturation in absence of salts Oryctolagus cuniculus

Inhibitors

Inhibitors Comment Organism Structure
2,3-Dimercaptopropanol competitive inhibitor to glycerophosphate Oryctolagus cuniculus
iodoacetate
-
Homo sapiens
iodoacetate 0.1 mM 23% inhibition Oryctolagus cuniculus
iodoacetate
-
Rattus norvegicus
additional information coenzyme analogs: acetylpyridine-NAD+, deaminoacetylpyridine-NAD+, pyridinealdehyde-NAD+, deaminopyridinealdehyde-NAD+, potent inhibitors Oryctolagus cuniculus
N-ethylmaleimide 0.1 mM complete inhibition Oryctolagus cuniculus
N-ethylmaleimide
-
Rattus norvegicus
p-mercuribenzoate 0.1 mM complete inhibition Oryctolagus cuniculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.11
-
alpha-glycerophosphate
-
Oryctolagus cuniculus
0.38
-
NAD+
-
Oryctolagus cuniculus
0.46
-
dihydroxyacetone phosphate
-
Oryctolagus cuniculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
Rattus norvegicus 5737
-
cytoplasm
-
Oryctolagus cuniculus 5737
-
cytoplasm
-
Locusta migratoria 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
78000
-
-
Rattus norvegicus
78000
-
calculated from partial specific volume, sedimentation and diffusion data Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Homo sapiens equilibrium far to the side of alpha-glycerophosphate at neutral pH dihydroxyacetone phosphate + NADH + H+
-
?
sn-glycerol 3-phosphate + NAD+ Rattus norvegicus equilibrium far to the side of alpha-glycerophosphate at neutral pH dihydroxyacetone phosphate + NADH + H+
-
?
sn-glycerol 3-phosphate + NAD+ Oryctolagus cuniculus equilibrium far to the side of alpha-glycerophosphate at neutral pH dihydroxyacetone phosphate + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-
Homo sapiens
-
-
-
Locusta migratoria
-
-
-
Mus musculus
-
-
-
Oryctolagus cuniculus
-
-
-
Rattus norvegicus
-
-
-

Reaction

Reaction Comment Organism Reaction ID
sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH + H+ one active site per enzyme molecule Homo sapiens
sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH + H+ one active site per enzyme molecule Rattus norvegicus
sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH + H+ one active site per enzyme molecule Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
breast cancer cell
-
Homo sapiens
-
kidney
-
Homo sapiens
-
kidney
-
Rattus norvegicus
-
leukocyte also other blood components Homo sapiens
-
leukocyte also other blood components Rattus norvegicus
-
liver
-
Homo sapiens
-
liver
-
Rattus norvegicus
-
liver
-
Oryctolagus cuniculus
-
muscle
-
Mus musculus
-
muscle
-
Rattus norvegicus
-
muscle
-
Oryctolagus cuniculus
-
muscle bovine smooth muscles Bos taurus
-
sarcoma cell
-
Homo sapiens
-
sarcoma cell
-
Oryctolagus cuniculus
-
sarcoma cell Crocker sarcoma Mus musculus
-

Storage Stability

Storage Stability Organism
4┬░C, dilute solution, pH 5.8, stable for weeks Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information
-
Oryctolagus cuniculus ?
-
?
sn-glycerol 3-phosphate + NAD+
-
Homo sapiens dihydroxyacetone phosphate + NADH + H+
-
?
sn-glycerol 3-phosphate + NAD+
-
Rattus norvegicus dihydroxyacetone phosphate + NADH + H+
-
?
sn-glycerol 3-phosphate + NAD+
-
Oryctolagus cuniculus dihydroxyacetone phosphate + NADH + H+
-
?
sn-glycerol 3-phosphate + NAD+ equilibrium far to the side of alpha-glycerophosphate at neutral pH Homo sapiens dihydroxyacetone phosphate + NADH + H+
-
?
sn-glycerol 3-phosphate + NAD+ equilibrium far to the side of alpha-glycerophosphate at neutral pH Rattus norvegicus dihydroxyacetone phosphate + NADH + H+
-
?
sn-glycerol 3-phosphate + NAD+ equilibrium far to the side of alpha-glycerophosphate at neutral pH Oryctolagus cuniculus dihydroxyacetone phosphate + NADH + H+
-
?

Temperature Stability [┬░C]

Temperature Stability Minimum [┬░C] Temperature Stability Maximum [┬░C] Comment Organism
20
-
stable for at least 30 min Oryctolagus cuniculus
55
-
t1/2: 1 min Oryctolagus cuniculus
60
-
-
Mus musculus
60
-
-
Homo sapiens
60
-
-
Rattus norvegicus
60
-
-
Bos taurus
60
-
complete inactivation after 1 min Oryctolagus cuniculus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
345
-
dihydroxyacetone phosphate reduction of synthetic dihydroxyacetone phosphate, 20┬░C, pH 7, rabbit muscle Oryctolagus cuniculus
390
-
dihydroxyacetone phosphate reduction of synthetic dihydroxyacetone phosphate, 20┬░C, pH 7, rabbit muscle, under conditions where competitive inhibition by fructose 1,6-diphosphate are not excluded Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
reduction of dihydroxyacetone phosphate, in 0.2 M phosphate buffer Oryctolagus cuniculus
10.2
-
oxidation of glycerol 3-phosphate, in 0.03 glycine buffer Oryctolagus cuniculus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.7 8.5 around this range, most stable Oryctolagus cuniculus
7
-
less stable Oryctolagus cuniculus

Cofactor

Cofactor Comment Organism Structure
additional information
-
Homo sapiens
additional information deamino-NAD+ 60.3% as effective as NAD+ Oryctolagus cuniculus
NAD+
-
Mus musculus
NAD+
-
Homo sapiens
NAD+
-
Rattus norvegicus
NAD+
-
Bos taurus
NAD+
-
Oryctolagus cuniculus
NADH
-
Mus musculus
NADH
-
Bos taurus
NADH 1 mol per mol enzyme Homo sapiens
NADH 1 mol per mol enzyme Rattus norvegicus
NADH 1 mol per mol enzyme Oryctolagus cuniculus