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Literature summary for 1.1.1.8 extracted from

  • Albertyn, J.; Van Tonder, A.; Prior, B.A.
    Purification and characterization of glycerol-3-phosphate dehydrogenase of Saccharomyces cerevisiae (1992), FEBS Lett., 308, 130-132.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
ADP at physiological concentration, 10 mM 90% inhibition Saccharomyces cerevisiae
ATP at physiological concentration, 10 mM 95% inhibition Saccharomyces cerevisiae
fructose-1,6-bisphosphate at physiological concentration, non-competitive Saccharomyces cerevisiae
additional information no inhibition by NADPH, acetaldehyde, glycerol, ethanol Saccharomyces cerevisiae
NAD+ competitive inhibitor to NADH at physiological concentration Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.023
-
NADH
-
Saccharomyces cerevisiae
0.54
-
dihydroxyacetone phosphate
-
Saccharomyces cerevisiae

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
42000
-
2 * 42000, SDS-PAGE Saccharomyces cerevisiae
68000
-
gel filtration Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Saccharomyces cerevisiae oxidation at 3% the reaction rate of dihydroxyacetone phosphate reduction at pH 7.0 dihydroxyacetone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+ Saccharomyces cerevisiae H44-3D oxidation at 3% the reaction rate of dihydroxyacetone phosphate reduction at pH 7.0 dihydroxyacetone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
-
-
Saccharomyces cerevisiae H44-3D
-
-
-

Purification (Commentary)

Purification (Comment) Organism
affinity chromatography Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
41.1
-
-
Saccharomyces cerevisiae

Storage Stability

Storage Stability Organism
-20┬░C, in 0.1 M Tris-HCl, 2 mM 2-mercaptoethanol, pH 7.0, 50% polyethylene glycol 2000, 5 mM NADH or dihydroxyacetone phosphate, unstable Saccharomyces cerevisiae
-80┬░C, in 0.1 M Tris-HCl, 2 mM 2-mercaptoethanol, pH 7.0, 50% polyethylene glycol 2000, 5 mM NADH or dihydroxyacetone phosphate, unstable Saccharomyces cerevisiae
4┬░C, 1 M Tris-HCl, 2 mM 2-mercaptoethanol, pH 7.0, eluted from Sephadex G-100 column, 90% activity remained after several weeks Saccharomyces cerevisiae
4┬░C, stable for several weeks in 1 M Tris/HCl buffer with 2-mercaptoethanol, pH 7.0 Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information glycerol Saccharomyces cerevisiae ?
-
?
additional information no substrates: dihydroxyacetone Saccharomyces cerevisiae ?
-
?
additional information glycerol Saccharomyces cerevisiae H44-3D ?
-
?
additional information no substrates: dihydroxyacetone Saccharomyces cerevisiae H44-3D ?
-
?
sn-glycerol 3-phosphate + NAD+
-
Saccharomyces cerevisiae dihydroxyacetone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+ oxidation at 3% the reaction rate of dihydroxyacetone phosphate reduction at pH 7.0 Saccharomyces cerevisiae dihydroxyacetone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+
-
Saccharomyces cerevisiae H44-3D dihydroxyacetone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+ oxidation at 3% the reaction rate of dihydroxyacetone phosphate reduction at pH 7.0 Saccharomyces cerevisiae H44-3D dihydroxyacetone phosphate + NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 42000, SDS-PAGE Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.6
-
maximum rate of dihydroxyacetone phosphate reduction Saccharomyces cerevisiae

pH Range

pH Minimum pH Maximum Comment Organism
7 8 dihydroxyacetone reduction, activity decreases rapidly below pH 7.0 and above pH 8.0, more rapidly at acidic values Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADPH Saccharomyces cerevisiae
NAD+
-
Saccharomyces cerevisiae
NADH
-
Saccharomyces cerevisiae