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Literature summary for 1.1.1.8 extracted from

  • Kloeck, G.; Kreuzberg, K.
    Kinetic properties of a sn-glycerol-3-phosphate dehydrogenase purified from the unicellular alga Chlamydomonas reinhardtii (1989), Biochim. Biophys. Acta, 991, 347-352.
    View publication on PubMed

General Stability

General Stability Organism
2-mercaptoethanol does not stabilize Chlamydomonas reinhardtii
20% polyethylene glycol stabilizes during purification Chlamydomonas reinhardtii
dithiothreitol does not stabilize Chlamydomonas reinhardtii
EDTA stabilizes Chlamydomonas reinhardtii

Inhibitors

Inhibitors Comment Organism Structure
Cl- competitive inhibitor with respect to dihydroxyacetone phosphate Chlamydomonas reinhardtii
dihydroxyacetone phosphate substrate inhibition above 1 mM, NADH above 0.2 mM; substrate inhibition only at high concentration, over 1 mM Chlamydomonas reinhardtii
L-glycerol 3-phosphate competitive inhibitor to dihydroxyacetone phosphate, non-competitive to NADH Chlamydomonas reinhardtii
N-ethylmaleimide 1 mM results in 50% inhibition, reversible by NADH, inhibition increases in the presence of dihydroxyacetone phosphate and/or glycerol 3-phosphate Chlamydomonas reinhardtii
NAD+ competitive inhibitor to NADH at physiological concentration; non-competitive to dihydroxyacetone phosphate Chlamydomonas reinhardtii
NADH at high concentration Chlamydomonas reinhardtii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.012
-
dihydroxyacetone phosphate
-
Chlamydomonas reinhardtii
0.016
-
NADH
-
Chlamydomonas reinhardtii

Localization

Localization Comment Organism GeneOntology No. Textmining
chloroplast stroma, soluble Chlamydomonas reinhardtii 9507
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
33000
-
x * 33000, calculated from Stokes radius Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
sn-glycerol 3-phosphate + NAD+ Chlamydomonas reinhardtii oxidation at 25% the rate of dihydroxyacetone phosphate reduction at optimal pH dihydroxyacetone phosphate + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Chlamydomonas reinhardtii
-
green alga
-
Chlamydomonas reinhardtii 11/32-90
-
green alga
-

Purification (Commentary)

Purification (Comment) Organism
dye-affinity chromatography Chlamydomonas reinhardtii

Reaction

Reaction Comment Organism Reaction ID
sn-glycerol 3-phosphate + NAD+ = glycerone phosphate + NADH + H+ equilibrium random-bi-bi reaction mechanism Chlamydomonas reinhardtii

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
34
-
-
Chlamydomonas reinhardtii

Storage Stability

Storage Stability Organism
-20┬░C, stable for at least 2 months Chlamydomonas reinhardtii
0┬░C, t1/2: 50 min Chlamydomonas reinhardtii
4┬░C, 20% v/v, glycerol, purified stable Chlamydomonas reinhardtii
4┬░C, t1/2: 6 h in absence of EDTA and polyethyleneglycol Chlamydomonas reinhardtii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information glyceraldehyde-3-phosphate Chlamydomonas reinhardtii ?
-
?
additional information fructose-6-phosphate, fructose-1,6-bisphosphate Chlamydomonas reinhardtii ?
-
?
additional information glucose-6-phosphate, acetaldehyde, oxaloacetate Chlamydomonas reinhardtii ?
-
?
additional information no substrates: dihydroxyacetone Chlamydomonas reinhardtii ?
-
?
additional information glyceraldehyde-3-phosphate Chlamydomonas reinhardtii 11/32-90 ?
-
?
additional information fructose-6-phosphate, fructose-1,6-bisphosphate Chlamydomonas reinhardtii 11/32-90 ?
-
?
additional information glucose-6-phosphate, acetaldehyde, oxaloacetate Chlamydomonas reinhardtii 11/32-90 ?
-
?
additional information no substrates: dihydroxyacetone Chlamydomonas reinhardtii 11/32-90 ?
-
?
sn-glycerol 3-phosphate + NAD+
-
Chlamydomonas reinhardtii dihydroxyacetone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+ oxidation at 25% the rate of dihydroxyacetone phosphate reduction at optimal pH Chlamydomonas reinhardtii dihydroxyacetone phosphate + NADH + H+
-
r
sn-glycerol 3-phosphate + NAD+
-
Chlamydomonas reinhardtii 11/32-90 dihydroxyacetone phosphate + NADH + H+
-
r

Subunits

Subunits Comment Organism
? x * 33000, calculated from Stokes radius Chlamydomonas reinhardtii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.8
-
dihydroxyacetone phosphate reduction Chlamydomonas reinhardtii
9.3
-
glycerol-phosphate oxidation Chlamydomonas reinhardtii

pH Stability

pH Stability pH Stability Maximum Comment Organism
additional information
-
increasing pH from 7.0 to 8.0 causes 80% loss of activity Chlamydomonas reinhardtii
7
-
stable, increasing from 7.0 to 8.0 causes 80% loss of activity Chlamydomonas reinhardtii

Cofactor

Cofactor Comment Organism Structure
additional information activity with dihydroxyacetone phosphate and NADPH 5% of that with NADH Chlamydomonas reinhardtii
NAD+
-
Chlamydomonas reinhardtii
NADH
-
Chlamydomonas reinhardtii