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Literature summary for 1.1.1.79 extracted from

  • Zarei, A.; Brikis, C.J.; Bajwa, V.S.; Chiu, G.Z.; Simpson, J.P.; DeEll, J.R.; Bozzo, G.G.; Shelp, B.J.
    Plant glyoxylate/succinic semialdehyde reductases comparative biochemical properties, function during chilling stress, and subcellular localization (2017), Front. Plant Sci., 8, 1399 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.79

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Oryza sativa
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana
expressed in Escherichia coli BL21(DE3) cells Malus domestica

Inhibitors

Inhibitors Comment Organism Structure
NADP+
-
 Arabidopsis thaliana
NADP+
-
 Malus domestica
NADP+
-
 Oryza sativa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0012
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
0.0014
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
0.0018
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.3 and 25°C Malus domestica
0.0022
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
0.0026
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
0.0033
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 7.5 and 25°C Malus domestica
0.0074
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 7.5 and 25°C Malus domestica
0.0084
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.1 and 25°C Oryza sativa
0.0088
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 6.5 and 25°C Oryza sativa
0.0117
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.3 and 25°C Malus domestica
0.0125
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.1 and 25°C Oryza sativa
0.0141
-
 glyoxylate isoform GLYR1, at pH 7.5 and 25°C Malus domestica
0.0191
-
 glyoxylate isoform GLYR2, at pH 7.1 and 25°C Oryza sativa
0.0193
-
 glyoxylate isoform GLYR2, at pH 7.8 and 25°C Arabidopsis thaliana
0.0232
-
 glyoxylate isoform GLYR1, at pH 7.8 and 25°C Arabidopsis thaliana
0.0239
-
 glyoxylate isoform GLYR2, at pH 7.3 and 25°C Malus domestica
0.0362
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 6.5 and 25°C Oryza sativa
0.0532
-
 glyoxylate isoform GLYR1, at pH 6.5 and 25°C Oryza sativa
0.87
-
 Succinic semialdehyde isoform GLYR1, at pH 7.8 and 25°C Arabidopsis thaliana
1.133
-
 Succinic semialdehyde isoform GLYR1, at pH 7.5 and 25°C Malus domestica
1.457
-
 Succinic semialdehyde isoform GLYR2, at pH 7.1 and 25°C Oryza sativa
4.003
-
 Succinic semialdehyde isoform GLYR1, at pH 6.5 and 25°C Oryza sativa
6.5
-
 Succinic semialdehyde isoform GLYR2, at pH 7.3 and 25°C Malus domestica
8.96
-
 Succinic semialdehyde isoform GLYR2, at pH 7.8 and 25°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol isoform GLYR1 Oryza sativa 5829
-
cytosol isoform GLYR1 Arabidopsis thaliana 5829
-
cytosol isoform GLYR1 Malus domestica 5829
-
mitochondrion isoform GLYR2 Oryza sativa 5739
-
mitochondrion isoform GLYR2 Arabidopsis thaliana 5739
-
mitochondrion isoform GLYR2 Malus domestica 5739
-
plastid isoform GLYR2 Oryza sativa 9536
-
plastid isoform GLYR2 Arabidopsis thaliana 9536
-
plastid isoform GLYR2 Malus domestica 9536
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glyoxylate + NADPH + H+ Oryza sativa
-
 glycolate + NADP+
-
 ?
glyoxylate + NADPH + H+ Arabidopsis thaliana
-
 glycolate + NADP+
-
 ?
glyoxylate + NADPH + H+ Malus domestica
-
 glycolate + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana F4I907
-
-
Arabidopsis thaliana Q9LSV0
-
-
Malus domestica A0A1C8M582
-
-
Malus domestica A0A1C8M593
-
-
Oryza sativa
-
-
-

Purification (Commentary)

Purification (Comment) Organism
nickel affinity column chromatography Oryza sativa
nickel affinity column chromatography Arabidopsis thaliana
nickel affinity column chromatography Malus domestica

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
 Malus domestica
-
leaf
-
 Oryza sativa
-
leaf
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glyoxylate + NADPH + H+
-
 Oryza sativa glycolate + NADP+
-
 ?
glyoxylate + NADPH + H+
-
 Arabidopsis thaliana glycolate + NADP+
-
 ?
glyoxylate + NADPH + H+
-
 Malus domestica glycolate + NADP+
-
 ?
glyoxylate + NADPH + H+ the enzyme prefers glyoxylate over succinic semialdehyde, and has a high affinity for their co-substrate NADPH Oryza sativa glycolate + NADP+
-
 ?
glyoxylate + NADPH + H+ the enzyme prefers glyoxylate over succinic semialdehyde, and has a high affinity for their co-substrate NADPH Arabidopsis thaliana glycolate + NADP+
-
 ?
glyoxylate + NADPH + H+ the enzyme prefers glyoxylate over succinic semialdehyde, and has a high affinity for their co-substrate NADPH Malus domestica glycolate + NADP+
-
 ?
succinic semialdehyde + NADPH + H+ the enzyme prefers glyoxylate over succinic semialdehyde, and has a high affinity for their co-substrate NADPH Oryza sativa 4-hydroxybutyrate + NADP+
-
 ?
succinic semialdehyde + NADPH + H+ the enzyme prefers glyoxylate over succinic semialdehyde, and has a high affinity for their co-substrate NADPH Arabidopsis thaliana 4-hydroxybutyrate + NADP+
-
 ?
succinic semialdehyde + NADPH + H+ the enzyme prefers glyoxylate over succinic semialdehyde, and has a high affinity for their co-substrate NADPH Malus domestica 4-hydroxybutyrate + NADP+
-
 ?

Subunits

Subunits Comment Organism
? x * 30500, isoform GLYR1, calculated from amino acid sequence Oryza sativa
? x * 30700, isoform GLYR1, calculated from amino acid sequence Arabidopsis thaliana
? x * 31400, isoform GLYR2, calculated from amino acid sequence Oryza sativa
? x * 31800, isoform GLYR1, calculated from amino acid sequence Malus domestica
? x * 33100, isoform GLYR2, calculated from amino acid sequence Malus domestica
? x * 33200, isoform GLYR2, calculated from amino acid sequence Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
glyoxylate/succinic semialdehyde reductase
-
 Oryza sativa
glyoxylate/succinic semialdehyde reductase
-
 Arabidopsis thaliana
glyoxylate/succinic semialdehyde reductase
-
 Malus domestica
GLYR1 isoform Oryza sativa
GLYR1 isoform Arabidopsis thaliana
GLYR1 isoform Malus domestica
GLYR2 isoform Oryza sativa
GLYR2 isoform Arabidopsis thaliana
GLYR2 isoform Malus domestica

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.6
-
 Succinic semialdehyde isoform GLYR1, at pH 7.5 and 25°C Malus domestica
5.5
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 7.5 and 25°C Malus domestica
6.5
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.1 and 25°C Oryza sativa
7.2
-
 Succinic semialdehyde isoform GLYR2, at pH 7.1 and 25°C Oryza sativa
8.1
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
8.6
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.3 and 25°C Malus domestica
8.9
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.1 and 25°C Oryza sativa
9.3
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
10.1
-
 Succinic semialdehyde isoform GLYR1, at pH 7.8 and 25°C Arabidopsis thaliana
10.3
-
 Succinic semialdehyde isoform GLYR2, at pH 7.3 and 25°C Malus domestica
10.7
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 6.5 and 25°C Oryza sativa
10.7
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
10.8
-
 glyoxylate isoform GLYR2, at pH 7.1 and 25°C Oryza sativa
12
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
14.2
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 6.5 and 25°C Oryza sativa
15.7
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.3 and 25°C Malus domestica
17
-
 Succinic semialdehyde isoform GLYR2, at pH 7.8 and 25°C Arabidopsis thaliana
18.4
-
 glyoxylate isoform GLYR2, at pH 7.8 and 25°C Arabidopsis thaliana
19.1
-
 glyoxylate isoform GLYR2, at pH 7.3 and 25°C Malus domestica
21.9
-
 Succinic semialdehyde isoform GLYR1, at pH 6.5 and 25°C Oryza sativa
22.3
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 7.5 and 25°C Malus domestica
28.4
-
 glyoxylate isoform GLYR1, at pH 7.8 and 25°C Arabidopsis thaliana
30.9
-
 glyoxylate isoform GLYR1, at pH 7.5 and 25°C Malus domestica
33.7
-
 glyoxylate isoform GLYR1, at pH 6.5 and 25°C Oryza sativa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
 isoform GLYR1 Oryza sativa
6.8
-
-
 Malus domestica
6.8
-
 isoform GLYR2 Oryza sativa

pH Range

pH Minimum pH Maximum Comment Organism
5.5 7 enzyme isoform GLYR1 retains at least half of maximal activity over the pH range of 5.5-7.0 Oryza sativa
5.5 7 enzyme isoform GLYR1 retains at least half of maximal activity over the pH range of 5.5-7.0 Malus domestica

Cofactor

Cofactor Comment Organism Structure
NADPH the highest catalytic efficiency is observed for NADPH Oryza sativa
NADPH the highest catalytic efficiency is observed for NADPH Arabidopsis thaliana
NADPH the highest catalytic efficiency is observed for NADPH Malus domestica

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.6
-
 Succinic semialdehyde isoform GLYR2, at pH 7.3 and 25°C Malus domestica
1.9
-
 Succinic semialdehyde isoform GLYR2, at pH 7.8 and 25°C Arabidopsis thaliana
5.1
-
 Succinic semialdehyde isoform GLYR1, at pH 7.5 and 25°C Malus domestica
5.1
-
 Succinic semialdehyde isoform GLYR2, at pH 7.1 and 25°C Oryza sativa
5.4
-
 Succinic semialdehyde isoform GLYR1, at pH 6.5 and 25°C Oryza sativa
11.6
-
 Succinic semialdehyde isoform GLYR1, at pH 7.8 and 25°C Arabidopsis thaliana
290
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 6.5 and 25°C Oryza sativa
349
-
 glyoxylate isoform GLYR2, at pH 7.1 and 25°C Oryza sativa
525
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.1 and 25°C Oryza sativa
636
-
 glyoxylate isoform GLYR1, at pH 6.5 and 25°C Oryza sativa
660
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
796
-
 glyoxylate isoform GLYR2, at pH 7.3 and 25°C Malus domestica
832
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 7.5 and 25°C Malus domestica
833
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.3 and 25°C Malus domestica
906
-
 glyoxylate isoform GLYR2, at pH 7.8 and 25°C Arabidopsis thaliana
1040
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.1 and 25°C Oryza sativa
1259
-
 glyoxylate isoform GLYR1, at pH 7.8 and 25°C Arabidopsis thaliana
1729
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 6.5 and 25°C Oryza sativa
2230
-
 glyoxylate isoform GLYR1, at pH 7.5 and 25°C Malus domestica
2870
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
3500
-
 NADPH isoform GLYR1, with succinic semialdehyde as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
6803
-
 NADPH isoform GLYR1, with glyoxylate as cosubstrate, at pH 7.5 and 25°C Malus domestica
9180
-
 NADPH isoform GLYR2, with succinic semialdehyde as cosubstrate, at pH 7.8 and 25°C Arabidopsis thaliana
10070
-
 NADPH isoform GLYR2, with glyoxylate as cosubstrate, at pH 7.3 and 25°C Malus domestica