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Literature summary for 1.1.1.67 extracted from

  • Krahulec, S.; Armao, G.C.; Klimacek, M.; Nidetzky, B.
    Enzymes of mannitol metabolism in the human pathogenic fungus Aspergillus fumigatus--kinetic properties of mannitol-1-phosphate 5-dehydrogenase and mannitol 2-dehydrogenase, and their physiological implications (2011), FEBS J., 278, 1264-1276.
    View publication on PubMed
Search for more literature for 1.1.1.67

Inhibitors

Inhibitors Comment Organism Structure
ADP
-
 Aspergillus fumigatus
AMP
-
 Aspergillus fumigatus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.015
-
 NADH pH 7.1, 25°C Aspergillus fumigatus
0.11
-
 NAD+ pH 7.1, 25°C Aspergillus fumigatus
0.6
-
 D-fructose pH 7.1, 25°C Aspergillus fumigatus
1.7
-
 D-xylulose pH 7.1, 25°C Aspergillus fumigatus
13
-
 D-mannitol pH 10.0, 25°C Aspergillus fumigatus
163
-
 D-arabinitol pH 10.0, 25°C Aspergillus fumigatus
680
-
 L-sorbitol pH 10.0, 25°C Aspergillus fumigatus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-fructose + NADH + H+ Aspergillus fumigatus
-
 D-mannitol + NAD+
-
 r
D-mannitol + NAD+ Aspergillus fumigatus free energy profiles for the enzymatic reaction suggest that enzyme primarily acts in D-mannitol oxidation D-fructose + NADH + H+
-
 r

Organism

Organism UniProt Comment Textmining
Aspergillus fumigatus Q4WQY4
-
-

Reaction

Reaction Comment Organism Reaction ID
D-mannitol + NAD+ = D-fructose + NADH + H+ binding of substrate and NAD(H) is random for both D-mannitol oxidation and D-fructose reduction. Hydride transfer is rate-determining for D-fructose reduction. Product release steps control the maximum rates in the other direction of the enzymatic reaction Aspergillus fumigatus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-arabinitol + NAD+
-
 Aspergillus fumigatus D-xylulose + NADH + H+
-
 r
D-fructose + NADH + H+
-
 Aspergillus fumigatus D-mannitol + NAD+
-
 r
D-mannitol + NAD+ free energy profiles for the enzymatic reaction suggest that enzyme primarily acts in D-mannitol oxidation Aspergillus fumigatus D-fructose + NADH + H+
-
 r
D-xylulose + NADH + H+
-
 Aspergillus fumigatus D-arabinitol + NAD+
-
 ?
L-sorbitol + NAD+
-
 Aspergillus fumigatus L-sorbose + NADH + H+
-
 r
L-sorbose + NADH + H+
-
 Aspergillus fumigatus L-sorbitol + NAD+
-
 r
additional information a D-arabo configuration is required for a polyol substrate to become reactive. The C2 (R) configuration as in mannitol is preferred over the C2 (S) configuration as in D-sorbitol Aspergillus fumigatus ?
-
 ?

Synonyms

Synonyms Comment Organism
M2DH
-
 Aspergillus fumigatus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
25
-
 half-life 3.6 h Aspergillus fumigatus
30
-
 half-life 0.42 h Aspergillus fumigatus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
60
-
 L-sorbitol pH 10.0, 25°C Aspergillus fumigatus
64
-
 D-xylulose pH 7.1, 25°C Aspergillus fumigatus
86
-
 D-fructose pH 7.1, 25°C Aspergillus fumigatus
162
-
 D-arabinitol pH 10.0, 25°C Aspergillus fumigatus
212
-
 D-mannitol pH 10.0, 25°C Aspergillus fumigatus

Cofactor

Cofactor Comment Organism Structure
additional information only trace activity for utilization of NADP+ Aspergillus fumigatus
NAD+
-
 Aspergillus fumigatus
NADH
-
 Aspergillus fumigatus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.9
-
 AMP pH 7.1, 25°C, reduction of fructose Aspergillus fumigatus
4.8
-
 AMP pH 7.1, 25°C, oxidation of mannitol Aspergillus fumigatus
5.6
-
 ADP pH 7.1, 25°C, reduction of fructose Aspergillus fumigatus
5.7
-
 ADP pH 7.1, 25°C, oxidation of mannitol Aspergillus fumigatus

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.088
-
 L-sorbitol pH 10.0, 25°C Aspergillus fumigatus
0.99
-
 D-arabinitol pH 10.0, 25°C Aspergillus fumigatus
1.1
-
 L-sorbose pH 7.1, 25°C Aspergillus fumigatus
17
-
 D-mannitol pH 10.0, 25°C Aspergillus fumigatus
39
-
 D-xylulose pH 7.1, 25°C Aspergillus fumigatus
140
-
 D-fructose pH 7.1, 25°C Aspergillus fumigatus