Cloned (Comment) | Organism |
---|---|
expression of GST-tagged wild-type and mutant enzymes in Escherichia coli strain JM107 | Curvularia lunata |
Protein Variants | Comment | Organism |
---|---|---|
A50R | site-directed mutagenesis, the mutant shows increased rates of NADPH dissociation and thus enhanced substrate oxidation with NADP+ compared to the wild-type enzyme | Curvularia lunata |
N51R | site-directed mutagenesis, the mutant shows increased rates of NADP+ dissociation and thus enhanced substrate reduction with NADPH compared to the wild-type enzyme | Curvularia lunata |
T202I | site-directed mutagenesis, inactive mutant due to cofactor binding deficiency | Curvularia lunata |
T202V | site-directed mutagenesis, inactive mutant due to cofactor binding deficiency | Curvularia lunata |
Y49D | site-directed mutagenesis, the mutant shows altered cofactor specificity preferring NAD+, resembling to the the cD1d subfamily of the short-chain dehydrogenase/reductase superfamily | Curvularia lunata |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics of wild-type and mutant enzymes, Theorell-Chance reaction mechanism | Curvularia lunata |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-estrene-3,17-dione + NADPH | Curvularia lunata | - |
4-estrene-17beta-ol-3-one + NADP+ | - |
r | |
estrone + NADPH | Curvularia lunata | - |
17beta-estradiol + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Curvularia lunata | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged wild-type and mutant enzymes from Escherichia coli strain JM107 by glutathione affinity chromatography | Curvularia lunata |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
17beta-estradiol + NAD(P)+ = estrone + NAD(P)H + H+ | cofactor binding residues are Tyr49, Thr202, Ala50, and Asn51, Theorell-Chance reaction mechanism | Curvularia lunata |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-estrene-3,17-dione + NADPH | - |
Curvularia lunata | 4-estrene-17beta-ol-3-one + NADP+ | - |
r | |
estrone + NADPH | - |
Curvularia lunata | 17beta-estradiol + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
17beta-HSD | - |
Curvularia lunata |
17beta-hydroxysteroid dehydrogenase | - |
Curvularia lunata |
More | the enzyme belongs to the cP1 subfamily of the short-chain dehydrogenase/reductase superfamily | Curvularia lunata |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Curvularia lunata |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Curvularia lunata |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | cofactor binding mechanism and specificity involving residues Tyr49, Thr202, Ala50, and Asn51, overview | Curvularia lunata | |
NAD+ | mutant Y49D, not the wild-type enzyme | Curvularia lunata | |
NADP+ | dependent on | Curvularia lunata | |
NADPH | dependent on | Curvularia lunata |