Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
104000 | - |
sucose density gradient centrifugation, gel filtration | Ovis aries |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Ovis aries | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Ovis aries |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
ovary | - |
Ovis aries | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.136 | - |
- |
Ovis aries |
Storage Stability | Organism |
---|---|
-85°C, most stable between pH 6.5 and 7.0 | Ovis aries |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-O-methyl-17beta-estradiol + NAD+ | 85% of the activity with estradiol-17beta | Ovis aries | 3-O-methylestrone + NADH | - |
? | |
estradiol-17beta + NAD+ | reversible at neutral pH, reduction of estrone at 5% of the activity of reduction of estradiol-17beta | Ovis aries | estrone + NADH | - |
r | |
estradiol-17beta + NADP+ | reversible at neutral pH, reduction of estrone at 5% of the activity of reduction of estradiol-17beta | Ovis aries | estrone + NADPH | - |
r | |
estradiol-17beta + NADP+ | 17% of the activity with NAD+ | Ovis aries | estrone + NADPH | - |
r | |
estrone + NADH | reversible at neutral pH, reduction of estrone at 5% of the activity of reduction of estradiol-17beta | Ovis aries | estradiol-17beta + NAD+ | - |
r | |
estrone + NADPH + H+ | reversible at neutral pH, reduction of estrone at 5% of the activity of oxidation of estradiol-17beta | Ovis aries | estradiol-17beta + NADP+ | - |
r |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
NADP+, NADPH, MnSO4 and (NH4)2SO4 are detrimental to stability | Ovis aries |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.2 | - |
estradiol-17beta + NAD+ | Ovis aries |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.7 | 9.2 | pH 6.7: about 45% of maximal activity, pH 9.2: about 60% of maximal activity | Ovis aries |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | cofactor | Ovis aries | |
NADH | cofactor | Ovis aries | |
NADP+ | cofactor | Ovis aries | |
NADP+ | 17% of the activity with NAD+ | Ovis aries | |
NADPH | cofactor | Ovis aries |