Literature summary for 1.1.1.6 extracted from

Sarma, S.; Ortega, D.; Minton, N.P.; Dubey, V.K.; Moholkar, V.S.
Homologous overexpression of hydrogenase and glycerol dehydrogenase in Clostridium pasteurianum to enhance hydrogen production from crude glycerol (2019), Biores. Technol., 284, 168-177 .

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Application

Application	Comment	Organism
synthesis	engineering of a hypertransformable variant of Clostridium pasteurianum for bioconversion of glycerol into hydrogen via increasing product yield by overexpression of enzyme catalyzing H2 production, and increasing substrate uptake by overexpression of enzymes involved in glycerol utilization. Overexpression of the HydA gene encoding hydrogenase, and overexpression of DhaD1 and DhaK genes encoding glycerol dehydrogenase and dihydroxyacetone kinase result in two recombinant strains (HydA++/HhaD1K++) capable of producing 97% H2 (v/v), with yields of 1.1 mol H2/mol glycerol in HydA overexpressed strain, and 0.93 mol H2/mol glycerol in DhaD1K overexpressing strain	Clostridium pasteurianum

Organism

Organism	UniProt	Comment	Textmining
Clostridium pasteurianum	A0A0H3J8E1	-	-
Clostridium pasteurianum DSM 525	A0A0H3J8E1	-	-

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Release 2023.1 (January 2023)