Literature summary for 1.1.1.50 extracted from

Yee, D.J.; Balsanek, V.; Sames, D.
New tools for molecular imaging of redox metabolism: development of a fluorogenic probe for 3a-hydroxysteroid dehydrogenases (2004), J. Am. Chem. Soc., 126, 2282-2283.

Search for more literature for 1.1.1.50

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.0025	-	oxidized 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one	isozyme AKR1C3	Homo sapiens
0.026	-	5alpha-dihydroprogesterone	isozyme AKR1C3	Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
5alpha-dihydroprogesterone + 2 NAD(P)H + 2 H+	Homo sapiens	-	pregnan-3alpha,20beta-diol + 2 NAD(P)+	-	?

Organism

Organism	UniProt	Comment	Textmining
Comamonas testosteroni	-	-	-
Homo sapiens	-	type 2 isozyme AKR1C3	-
Rattus norvegicus	-	-	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
additional information	-	developemnt of noninvasive method for visualization and quantification of the reaction, development of selective fluorogenic substrate compounds, overview	Rattus norvegicus
additional information	-	developemnt of noninvasive method for visualization and quantification of the reaction, development of selective fluorogenic substrate compounds, overview	Comamonas testosteroni
additional information	-	development of noninvasive method for visualization and quantification of the reaction, development of selective fluorogenic substrate compounds, overview	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
5alpha-dihydroprogesterone + 2 NAD(P)H + 2 H+	-	Homo sapiens	pregnan-3alpha,20beta-diol + 2 NAD(P)+	-	?
oxidized 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one + NAD(P)H	synthetic compound with fluorophore core	Homo sapiens	reduced 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one + NAD(P)+	-	r
oxidized 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one + NAD(P)H	synthetic compound with fluorophore core	Rattus norvegicus	reduced 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one + NAD(P)+	-	r
oxidized 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one + NAD(P)H	synthetic compound with fluorophore core	Comamonas testosteroni	reduced 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one + NAD(P)+	-	r

Synonyms

Synonyms	Comment	Organism
3alpha-hydroxysteroid dehydrogenase	-	Homo sapiens
3alpha-hydroxysteroid dehydrogenase	-	Rattus norvegicus
3alpha-hydroxysteroid dehydrogenase	-	Comamonas testosteroni
3alphaHSD	-	Homo sapiens
3HSD	-	Rattus norvegicus
3HSD	-	Comamonas testosteroni
AKR1C3	-	Homo sapiens
More	enzyme belongs to the aldo-keto reductase AKR superfamily	Homo sapiens
PT3HSD	-	Comamonas testosteroni

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.004	-	5alpha-dihydroprogesterone	isozyme AKR1C3	Homo sapiens
0.14	-	oxidized 8-acetyl-2,3,5,6-tetrahydro-1H,4H-11-oxa-3a-azabenzo[de]anthracen-10-one	isozyme AKR1C3	Homo sapiens

Cofactor

Cofactor	Comment	Organism	Structure
NAD(P)+	-	Homo sapiens
NAD(P)+	-	Rattus norvegicus
NAD(P)+	-	Comamonas testosteroni
NAD(P)H	-	Homo sapiens
NAD(P)H	-	Rattus norvegicus
NAD(P)H	-	Comamonas testosteroni

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Release 2023.1 (January 2023)