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Literature summary for 1.1.1.50 extracted from

  • Biswas, M.G.; Russel, D.W.
    Expression cloning and characterization of oxidative 17beta- and 3alpha-hydroxysteroid dehydrogenase from rat and human prostate (1997), J. Biol. Chem., 272, 15959-15966.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Homo sapiens
expression in Escherichia coli Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0001
-
androsterone
-
Homo sapiens
0.0001
-
androsterone
-
Rattus norvegicus
0.0001
-
5alpha-dihydrotestosterone
-
Homo sapiens
0.0001
-
3alpha-adiol
-
Rattus norvegicus
0.0028
-
testosterone
-
Rattus norvegicus
0.0033
-
testosterone
-
Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5alpha-dihydrotestosterone + NAD(P)H + H+ Homo sapiens discussion of physiological role 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
r
5alpha-dihydrotestosterone + NAD(P)H + H+ Rattus norvegicus discussion of physiological role 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
r

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
liver
-
Homo sapiens
-
liver
-
Rattus norvegicus
-
prostate gland
-
Homo sapiens
-
prostate gland
-
Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.005
-
-
Rattus norvegicus
0.011
-
-
Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3alpha-adiol + NAD(P)+
-
Homo sapiens dihydrotestosterone + NAD(P)H
-
r
3alpha-adiol + NAD(P)+
-
Rattus norvegicus dihydrotestosterone + NAD(P)H
-
r
5alpha-dihydrotestosterone + NAD(P)H + H+
-
Homo sapiens 5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
r
5alpha-dihydrotestosterone + NAD(P)H + H+
-
Rattus norvegicus 5alpha-androstan-3alpha,17beta-diol + NAD(P)+
-
r
5alpha-dihydrotestosterone + NAD(P)H + H+
-
Homo sapiens (3beta,5alpha,17beta)-androstane-3,17-diol + NAD(P)+
-
r
5alpha-dihydrotestosterone + NAD(P)H + H+
-
Rattus norvegicus (3beta,5alpha,17beta)-androstane-3,17-diol + NAD(P)+
-
r
5alpha-dihydrotestosterone + NAD(P)H + H+ discussion of physiological role Homo sapiens 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
r
5alpha-dihydrotestosterone + NAD(P)H + H+ discussion of physiological role Rattus norvegicus 5alpha-androstane-3alpha,17beta-diol + NAD(P)+
-
r
5beta-dihydrotestosterone + NAD(P)H
-
Homo sapiens 5beta-androstan-3alpha,17beta-diol + NAD(P)+
-
?
5beta-dihydrotestosterone + NAD(P)H
-
Rattus norvegicus 5beta-androstan-3alpha,17beta-diol + NAD(P)+
-
?
androsterone + NAD(P)+
-
Homo sapiens 5alpha-androstan-3,17-dione + NAD(P)H
-
r
androsterone + NAD(P)+
-
Rattus norvegicus 5alpha-androstan-3,17-dione + NAD(P)H
-
r
estradiol + NAD(P)H
-
Homo sapiens estrone + NAD(P)+
-
?
estradiol + NAD(P)H
-
Rattus norvegicus estrone + NAD(P)+
-
?
testosterone + NAD(P)+
-
Homo sapiens androstenedione + NAD(P)H + H+
-
?
testosterone + NAD(P)+
-
Rattus norvegicus androstenedione + NAD(P)H + H+
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0167
-
stanolone
-
Homo sapiens
0.0333
-
androsterone
-
Rattus norvegicus
0.0833
-
3alpha-adiol
-
Rattus norvegicus
0.133
-
androsterone
-
Homo sapiens
0.183
-
3alpha-adiol
-
Homo sapiens