KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.32 | - |
cyclohexanecarboxaldehyde | pH 7, 25°C | [Candida] intermedia | |
0.4 | - |
5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | Saitozyma flava | |
0.6 | - |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | Saitozyma flava | |
0.6 | - |
oenanthaldehyde | pH 7, 25°C | Saitozyma flava | |
0.7 | - |
5-deoxy-D-xylofuranose | pH 7, 25°C | Saitozyma flava | |
0.9 | - |
5,6-dideoxy-D-xylo-hexofuranose | pH 7, 25°C | Saitozyma flava | |
1 | - |
5-deoxy-D-xylofuranose | pH 7, 25°C | [Candida] intermedia | |
1 | - |
5-fluoro-5-deoxy-D-xylofuranose | pH 7, 25°C | Saitozyma flava | |
1 | - |
5,6-dideoxy-6-fluoro-D-xylo-hexofuranose | pH 7, 25°C | Saitozyma flava | |
1.3 | - |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
1.5 | - |
5,6-dideoxy-5,6-difluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
1.6 | - |
5-fluoro-5-deoxy-D-xylofuranose | pH 7, 25°C | [Candida] intermedia | |
2.2 | - |
5,6-dideoxy-D-xylo-hexofuranose | pH 7, 25°C | [Candida] intermedia | |
2.5 | - |
oenanthaldehyde | pH 7, 25°C | [Candida] intermedia | |
3 | - |
5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
4 | - |
5-azido-5-deoxy-D-xylofuranose | pH 7, 25°C | Saitozyma flava | |
4.4 | - |
5,6-dideoxy-5,6-difluoro-D-glucofuranose | pH 7, 25°C | Saitozyma flava | |
9.8 | - |
cyclohexanecarboxaldehyde | pH 7, 25°C | Saitozyma flava | |
10.4 | - |
5,6-dideoxy-6-fluoro-D-xylo-hexofuranose | pH 7, 25°C | [Candida] intermedia | |
27 | - |
D-xylose | pH 7, 25°C | Saitozyma flava | |
51 | - |
D-galactose | pH 7, 25°C | Saitozyma flava | |
82 | - |
D-xylose | pH 7, 25°C | [Candida] intermedia | |
303 | - |
D-galactose | pH 7, 25°C | [Candida] intermedia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADPH + H+ | Saitozyma flava | - |
xylitol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saitozyma flava | - |
- |
- |
[Candida] intermedia | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5,6-dideoxy-5,6-difluoro-D-glucofuranose + NADPH + H+ | - |
[Candida] intermedia | ? + NADP+ | - |
? | |
5,6-dideoxy-5,6-difluoro-D-glucofuranose + NADPH + H+ | - |
Saitozyma flava | ? + NADP+ | - |
? | |
5,6-dideoxy-5-fluoro-D-glucofuranose + NADPH + H+ | - |
[Candida] intermedia | ? + NADP+ | - |
? | |
5,6-dideoxy-5-fluoro-D-glucofuranose + NADPH + H+ | - |
Saitozyma flava | ? + NADP+ | - |
? | |
5,6-dideoxy-6-fluoro-D-xylo-hexofuranose + NADPH + H+ | - |
[Candida] intermedia | ? + NADP+ | - |
? | |
5,6-dideoxy-6-fluoro-D-xylo-hexofuranose + NADPH + H+ | - |
Saitozyma flava | ? + NADP+ | - |
? | |
5,6-dideoxy-D-xylo-hexofuranose + NADPH + H+ | - |
[Candida] intermedia | ? + NADP+ | - |
? | |
5,6-dideoxy-D-xylo-hexofuranose + NADPH + H+ | - |
Saitozyma flava | ? + NADP+ | - |
? | |
5-azido-5-deoxy-D-xylofuranose + NADPH + H+ | - |
[Candida] intermedia | ? + NADP+ | - |
? | |
5-azido-5-deoxy-D-xylofuranose + NADPH + H+ | - |
Saitozyma flava | ? + NADP+ | - |
? | |
5-deoxy-D-xylofuranose + NADPH + H+ | - |
[Candida] intermedia | ? + NADP+ | - |
? | |
5-deoxy-D-xylofuranose + NADPH + H+ | - |
Saitozyma flava | ? + NADP+ | - |
? | |
5-fluoro-5-deoxy-D-xylofuranose + NADPH + H+ | - |
[Candida] intermedia | ? + NADP+ | - |
? | |
5-fluoro-5-deoxy-D-xylofuranose + NADPH + H+ | - |
Saitozyma flava | ? + NADP+ | - |
? | |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose + NADPH + H+ | - |
[Candida] intermedia | ? + NADP+ | - |
? | |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose + NADPH + H+ | - |
Saitozyma flava | ? + NADP+ | - |
? | |
6-azido-5,6-dideoxy-D-xylo-hexofuranose + NADPH + H+ | - |
[Candida] intermedia | ? + NADP+ | - |
? | |
6-azido-5,6-dideoxy-D-xylo-hexofuranose + NADPH + H+ | - |
Saitozyma flava | ? + NADP+ | - |
? | |
cyclohexanecarboxaldehyde + NADPH + H+ | - |
[Candida] intermedia | ? + NADP+ | - |
? | |
cyclohexanecarboxaldehyde + NADPH + H+ | - |
Saitozyma flava | ? + NADP+ | - |
? | |
D-galactose + NADPH + H+ | - |
[Candida] intermedia | ? + NADP+ | - |
? | |
D-galactose + NADPH + H+ | - |
Saitozyma flava | ? + NADP+ | - |
? | |
D-xylose + NADPH + H+ | - |
[Candida] intermedia | xylitol + NADP+ | - |
? | |
D-xylose + NADPH + H+ | - |
Saitozyma flava | xylitol + NADP+ | - |
? | |
oenanthaldehyde + NADPH + H+ | - |
[Candida] intermedia | ? + NADP+ | - |
? | |
oenanthaldehyde + NADPH + H+ | - |
Saitozyma flava | ? + NADP+ | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5.9 | - |
D-galactose | pH 7, 25°C | Saitozyma flava | |
6.3 | - |
cyclohexanecarboxaldehyde | pH 7, 25°C | Saitozyma flava | |
8.8 | - |
D-xylose | pH 7, 25°C | Saitozyma flava | |
9.2 | - |
oenanthaldehyde | pH 7, 25°C | Saitozyma flava | |
9.4 | - |
D-galactose | pH 7, 25°C | [Candida] intermedia | |
11.2 | - |
5-azido-5-deoxy-D-xylofuranose | pH 7, 25°C | Saitozyma flava | |
12.5 | - |
5-deoxy-D-xylofuranose | pH 7, 25°C | Saitozyma flava | |
13.1 | - |
cyclohexanecarboxaldehyde | pH 7, 25°C | [Candida] intermedia | |
14 | - |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | Saitozyma flava | |
14 | - |
5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | Saitozyma flava | |
14 | - |
5,6-dideoxy-6-fluoro-D-xylo-hexofuranose | pH 7, 25°C | Saitozyma flava | |
14.6 | - |
D-xylose | pH 7, 25°C | [Candida] intermedia | |
15 | - |
5,6-dideoxy-D-xylo-hexofuranose | pH 7, 25°C | Saitozyma flava | |
15.6 | - |
5-fluoro-5-deoxy-D-xylofuranose | pH 7, 25°C | Saitozyma flava | |
18.1 | - |
5-deoxy-D-xylofuranose | pH 7, 25°C | [Candida] intermedia | |
19 | - |
5,6-dideoxy-5,6-difluoro-D-glucofuranose | pH 7, 25°C | Saitozyma flava | |
19 | - |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
20 | - |
5-fluoro-5-deoxy-D-xylofuranose | pH 7, 25°C | [Candida] intermedia | |
21 | - |
5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
22 | - |
5,6-dideoxy-5,6-difluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
22 | - |
oenanthaldehyde | pH 7, 25°C | [Candida] intermedia | |
27 | - |
5,6-dideoxy-D-xylo-hexofuranose | pH 7, 25°C | [Candida] intermedia | |
34 | - |
5,6-dideoxy-6-fluoro-D-xylo-hexofuranose | pH 7, 25°C | [Candida] intermedia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | enzyme is strictly specific for NADPH | Saitozyma flava | |
NADPH | isoform I is strictly specific for NADPH | [Candida] intermedia |
General Information | Comment | Organism |
---|---|---|
metabolism | derivatives of D-xylose and D-glucose, in which the hydroxy groups at C-5, and C-5 and C-6 are replaced by fluorine, hydrogen and azide are reduced with up to 3000fold increased catalytic efficiencies. Azide introduced at C-5 or C-6 destabilizes the transition state of reduction of the corresponding hydrogen-substituted aldoses by approx. 4 kJ/mol | [Candida] intermedia |
metabolism | derivatives of D-xylose and D-glucose, in which the hydroxy groups at C-5, and C-5 and C-6 are replaced by fluorine, hydrogen and azide are reduced with up to 3000fold increased catalytic efficiencies. Azide introduced at C-5 or C-6 destabilizes the transition state of reduction of the corresponding hydrogen-substituted aldoses by approx. 4 kJ/mol | Saitozyma flava |
physiological function | Candida intermedia produces two different isoforms. Isoform I is strictly specific for NADPH, isoform II shows similar specificity constants for NADPH and NADH | [Candida] intermedia |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
D-galactose | pH 7, 25°C | [Candida] intermedia | |
0.116 | - |
D-galactose | pH 7, 25°C | Saitozyma flava | |
0.18 | - |
D-xylose | pH 7, 25°C | [Candida] intermedia | |
0.2 | - |
6-azido-5,6-dideoxy-D-xylo-hexofuranose | pH 7, 25°C | [Candida] intermedia | |
0.33 | - |
D-xylose | pH 7, 25°C | Saitozyma flava | |
0.64 | - |
cyclohexanecarboxaldehyde | pH 7, 25°C | Saitozyma flava | |
0.9 | - |
6-azido-5,6-dideoxy-D-xylo-hexofuranose | pH 7, 25°C | Saitozyma flava | |
1.12 | - |
5-azido-5-deoxy-D-xylofuranose | pH 7, 25°C | [Candida] intermedia | |
2.8 | - |
5-azido-5-deoxy-D-xylofuranose | pH 7, 25°C | Saitozyma flava | |
3.27 | - |
5,6-dideoxy-6-fluoro-D-xylo-hexofuranose | pH 7, 25°C | [Candida] intermedia | |
4.32 | - |
5,6-dideoxy-5,6-difluoro-D-glucofuranose | pH 7, 25°C | Saitozyma flava | |
7 | - |
5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
8.8 | - |
oenanthaldehyde | pH 7, 25°C | [Candida] intermedia | |
12.27 | - |
5,6-dideoxy-D-xylo-hexofuranose | pH 7, 25°C | [Candida] intermedia | |
12.5 | - |
5-fluoro-5-deoxy-D-xylofuranose | pH 7, 25°C | [Candida] intermedia | |
14 | - |
5,6-dideoxy-6-fluoro-D-xylo-hexofuranose | pH 7, 25°C | Saitozyma flava | |
14.62 | - |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
14.67 | - |
5,6-dideoxy-5,6-difluoro-D-glucofuranose | pH 7, 25°C | [Candida] intermedia | |
15.33 | - |
oenanthaldehyde | pH 7, 25°C | Saitozyma flava | |
15.6 | - |
5-fluoro-5-deoxy-D-xylofuranose | pH 7, 25°C | Saitozyma flava | |
16.67 | - |
5,6-dideoxy-D-xylo-hexofuranose | pH 7, 25°C | Saitozyma flava | |
17.86 | - |
5-deoxy-D-xylofuranose | pH 7, 25°C | Saitozyma flava | |
18.1 | - |
5-deoxy-D-xylofuranose | pH 7, 25°C | [Candida] intermedia | |
23.33 | - |
6-azido-5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | Saitozyma flava | |
35 | - |
5,6-dideoxy-5-fluoro-D-glucofuranose | pH 7, 25°C | Saitozyma flava | |
40.94 | - |
cyclohexanecarboxaldehyde | pH 7, 25°C | [Candida] intermedia | |
400 | - |
NADPH | pH 7, 25°C | [Candida] intermedia |