Cloned (Comment) | Organism |
---|---|
gene XYL1 | Corynebacterium glutamicum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | production of xylitol from D-xylose and D-glucose with recombinant Corynebacterium glutamicum, the strain is engineered to express the xylose reductase gene XYL1of Pichia stipitis, and produces xylose reductase with a specific activity of ca. 0.6 U/mg protein. Due to the absence of xylose isomerase and xylitol dehydrogenase genes, loose catabolite repression, high NADPH regeneration capacity, and tolerance against sugar-induced osmotic stress, the recombinant biocatalyst is able to efficiently produce xylitol from D-xylose using glucose as source of reducing equivalents | Corynebacterium glutamicum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADPH + H+ | Corynebacterium glutamicum | - |
xylitol + NADP+ | - |
? | |
D-xylose + NADPH + H+ | Corynebacterium glutamicum ATCC 13032 | - |
xylitol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Corynebacterium glutamicum | - |
gene XYL1 | - |
Corynebacterium glutamicum ATCC 13032 | - |
gene XYL1 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADPH + H+ | - |
Corynebacterium glutamicum | xylitol + NADP+ | - |
? | |
D-xylose + NADPH + H+ | - |
Corynebacterium glutamicum ATCC 13032 | xylitol + NADP+ | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Corynebacterium glutamicum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Corynebacterium glutamicum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Corynebacterium glutamicum |