Cloned (Comment) | Organism |
---|---|
expression of the mutant xylulose reductase from Candida tenuis in Saccharomyces cerevisiae, co-expression with an engineered xylitol dehydrogenase, with altered cofactor specificity, from Galactocandida mastotermitis, the transformed strain shows up to 50% decreased glycerol yield without increase in ethanol during xylose fermentation, overview | Yamadazyma tenuis |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the mutant Candida tenuis enzyme is modified in its cofactor specificity showing preference for NADPH compared to NADH in the D-xylose reduction reaction, genetic metabolic engineering for improvement of xylose metabolism and fermentation in wild-type Saccharomyces cerevisiae strains, which are not able to naturally metabolize D-xylulose, overview | Yamadazyma tenuis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NAD(P)H + H+ | Yamadazyma tenuis | - |
xylitol + NAD(P)+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Yamadazyma tenuis | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.16 | 0.4 | recombinant enzyme in transgenic strains of Saccharomyces cerevisiae | Yamadazyma tenuis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NAD(P)H + H+ | - |
Yamadazyma tenuis | xylitol + NAD(P)+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
NAD(P)H-dependent xylose reductase | - |
Yamadazyma tenuis |
xylose reductase | - |
Yamadazyma tenuis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD(P)H | dependent on, the wild-type enzyme prefers NADH, while a modified mutant enzyme prefers NADPH in the D-xylose reduction reaction | Yamadazyma tenuis |