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Literature summary for 1.1.1.428 extracted from

  • Hilbert, B.J.; Grossman, S.R.; Schiffer, C.A.; Royer, W.E.
    Crystal structures of human CtBP in complex with substrate MTOB reveal active site features useful for inhibitor design (2014), FEBS Lett., 588, 1743-1748 .
    View publication on PubMedView publication on EuropePMC
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21-CodonPlus (DE3)-RILcells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
CtBP1 in complex with 4-(methylsulfanyl)-2-oxobutanoate and NAD+, hanging drop vapor diffusion method, using 200-300 mM magnesium chloride, 0-140 mM sodium formate, 100 mM HEPES pH 7.5, and 2.5 mM NAD+ Homo sapiens
CtBP2 in complex with 4-(methylsulfanyl)-2-oxobutanoate and NAD+, hanging drop vapor diffusion method, using 200 mM, potassium nitrate, 15-20% PEG 3350, and 100 mM bis-tris-propane pH 7.0 Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
4-(methylsulfanyl)-2-oxobutanoate substrate inhibition; substrate inhibition Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4-(methylsulfanyl)-2-oxobutanoate + NADH + H+ Homo sapiens
-
 (2R)-2-hydroxy-4-(methylsulfanyl)butanoate + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P56545
-
-
Homo sapiens Q13363
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography and Superdex 75 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-(methylsulfanyl)-2-oxobutanoate + NADH + H+
-
 Homo sapiens (2R)-2-hydroxy-4-(methylsulfanyl)butanoate + NAD+
-
 ?

Synonyms

Synonyms Comment Organism
C-terminal-binding protein 1
-
 Homo sapiens
C-terminal-binding protein 2
-
 Homo sapiens
CTBP1
-
 Homo sapiens
CtBP2
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Homo sapiens