Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.425 extracted from

  • Kuritani, Y.; Sato, K.; Dohra, H.; Umemura, S.; Kitaoka, M.; Fushinobu, S.; Yoshida, N.
    Conversion of levoglucosan into glucose by the coordination of four enzymes through oxidation, elimination, hydration, and reduction (2020), Sci. Rep., 10, 20066 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus smithii

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-dehydrolevoglucosan + NADH + H+ Bacillus smithii
-
levoglucosan + NAD+
-
r
3-dehydrolevoglucosan + NADH + H+ Bacillus smithii S-2701M
-
levoglucosan + NAD+
-
r
levoglucosan + NAD+ Bacillus smithii
-
3-dehydrolevoglucosan + NADH + H+
-
r
levoglucosan + NAD+ Bacillus smithii S-2701M
-
3-dehydrolevoglucosan + NADH + H+
-
r

Organism

Organism UniProt Comment Textmining
Bacillus smithii
-
-
-
Bacillus smithii S-2701M
-
-
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.32
-
substrate levoglucosan, pH 7.5, 30°C Bacillus smithii
6
-
substrate 3-dehydrolevoglucosan, pH 7.5, 30°C Bacillus smithii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-dehydrolevoglucosan + NADH + H+
-
Bacillus smithii levoglucosan + NAD+
-
r
3-dehydrolevoglucosan + NADH + H+
-
Bacillus smithii S-2701M levoglucosan + NAD+
-
r
levoglucosan + NAD+
-
Bacillus smithii 3-dehydrolevoglucosan + NADH + H+
-
r
levoglucosan + NAD+
-
Bacillus smithii S-2701M 3-dehydrolevoglucosan + NADH + H+
-
r

Subunits

Subunits Comment Organism
? x * 44000, SDS-PAGE Bacillus smithii

Synonyms

Synonyms Comment Organism
LgdA
-
Bacillus smithii
LgdH
-
Bacillus smithii

General Information

General Information Comment Organism
metabolism in the metabolic pathway from levoglucosan to glucose, LgdA (LGDH) catalyzes 3-dehydrogenation of levoglucosan to produce 3-keto-levoglucosan, which undergoes beta-elimination of 3-keto-levoglucosan by LgdB1, followed by hydration to produce 3-keto-D-glucose by LgdB2. Next, LgdC reduces 3-keto-D-glucose to glucose Bacillus smithii