Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Stenotrophomonas maltophilia |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.024 | - |
NADP+ | at pH 8.0 and 25°C | Stenotrophomonas maltophilia | |
0.026 | - |
NADPH | at pH 8.0 and 25°C | Stenotrophomonas maltophilia | |
0.031 | - |
syn-2-decyl-3-hydroxytetradecanoic acid | at pH 8.0 and 25°C | Stenotrophomonas maltophilia | |
0.161 | - |
(2R/2S)-2-hexyl-3-oxodecanoic acid | at pH 8.0 and 25°C | Stenotrophomonas maltophilia | |
0.181 | - |
(2R,3S)-2-hexyl-3-hydroxydecanoic acid | at pH 8.0 and 25°C | Stenotrophomonas maltophilia | |
0.202 | - |
syn-2-hexyl-3-hydroxydecanoic acid | at pH 8.0 and 25°C | Stenotrophomonas maltophilia | |
0.214 | - |
syn-2-octyl-3-hydroxydodecanoic acid | at pH 8.0 and 25°C | Stenotrophomonas maltophilia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
77124 | - |
His6-tagged enzyme, calculated from amino acid sequence | Stenotrophomonas maltophilia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+ | Stenotrophomonas maltophilia | - |
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+ | - |
r | |
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+ | Stenotrophomonas maltophilia K279a | - |
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+ | - |
r | |
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+ | Stenotrophomonas maltophilia | - |
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+ | - |
r | |
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+ | Stenotrophomonas maltophilia K279a | - |
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Stenotrophomonas maltophilia | B2FI29 | - |
- |
Stenotrophomonas maltophilia K279a | B2FI29 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-agarose affinity column chromatography | Stenotrophomonas maltophilia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+ | - |
Stenotrophomonas maltophilia | (R)-2-alkyl-3-oxoalkanoate + NADPH + H+ | - |
r | |
(2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+ | - |
Stenotrophomonas maltophilia K279a | (R)-2-alkyl-3-oxoalkanoate + NADPH + H+ | - |
r | |
(2R,3S)-2-hexyl-3-hydroxydecanoic acid + NADPH + H+ | - |
Stenotrophomonas maltophilia | ? | - |
? | |
(2R,3S)-2-hexyl-3-hydroxydecanoic acid + NADPH + H+ | - |
Stenotrophomonas maltophilia K279a | ? | - |
? | |
(2R/2S)-2-hexyl-3-oxodecanoic acid + NADPH + H+ | 13% activity compared to syn-2-decyl-3-hydroxytetradecanoic acid | Stenotrophomonas maltophilia | ? | - |
? | |
(2R/2S)-2-hexyl-3-oxodecanoic acid + NADPH + H+ | 13% activity compared to syn-2-decyl-3-hydroxytetradecanoic acid | Stenotrophomonas maltophilia K279a | ? | - |
? | |
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+ | - |
Stenotrophomonas maltophilia | (2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+ | - |
r | |
(R)-2-alkyl-3-oxoalkanoate + NADPH + H+ | - |
Stenotrophomonas maltophilia K279a | (2R,3S)-2-alkyl-3-hydroxyalkanoate + NADP+ | - |
r | |
additional information | no activity with syn-2-butyl-3-hydroxyoctanoic acid, 3-oxodecanoic acid, and 3-hydroxydecanoic acid | Stenotrophomonas maltophilia | ? | - |
? | |
additional information | no activity with syn-2-butyl-3-hydroxyoctanoic acid, 3-oxodecanoic acid, and 3-hydroxydecanoic acid | Stenotrophomonas maltophilia K279a | ? | - |
? | |
syn-2-decyl-3-hydroxytetradecanoic acid + NADPH + H+ | 100% activity | Stenotrophomonas maltophilia | ? | - |
? | |
syn-2-hexyl-3-hydroxydecanoic acid + NADPH + H+ | 15% activity compared to syn-2-decyl-3-hydroxytetradecanoic acid | Stenotrophomonas maltophilia | ? | - |
? | |
syn-2-octyl-3-hydroxydodecanoic acid + NADPH + H+ | 20% activity compared to syn-2-decyl-3-hydroxytetradecanoic acid | Stenotrophomonas maltophilia | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 36000, SDS-PAGE | Stenotrophomonas maltophilia |
homodimer | 2 * 36472, calculated from amino acid sequence | Stenotrophomonas maltophilia |
Synonyms | Comment | Organism |
---|---|---|
2-alkyl-3-ketoalkanoic acid reductase | - |
Stenotrophomonas maltophilia |
oleD | - |
Stenotrophomonas maltophilia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.97 | - |
(2R/2S)-2-hexyl-3-oxodecanoic acid | at pH 8.0 and 25°C | Stenotrophomonas maltophilia | |
1.2 | - |
syn-2-hexyl-3-hydroxydecanoic acid | at pH 8.0 and 25°C | Stenotrophomonas maltophilia | |
1.3 | - |
(2R,3S)-2-hexyl-3-hydroxydecanoic acid | at pH 8.0 and 25°C | Stenotrophomonas maltophilia | |
1.4 | - |
syn-2-decyl-3-hydroxytetradecanoic acid | at pH 8.0 and 25°C | Stenotrophomonas maltophilia | |
2 | - |
syn-2-octyl-3-hydroxydodecanoic acid | at pH 8.0 and 25°C | Stenotrophomonas maltophilia |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Stenotrophomonas maltophilia | |
NADPH | - |
Stenotrophomonas maltophilia |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is involved in olefin biosynthesis | Stenotrophomonas maltophilia |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
(2R/2S)-2-hexyl-3-oxodecanoic acid | at pH 8.0 and 25°C | Stenotrophomonas maltophilia | |
6 | - |
syn-2-hexyl-3-hydroxydecanoic acid | at pH 8.0 and 25°C | Stenotrophomonas maltophilia | |
7.3 | - |
(2R,3S)-2-hexyl-3-hydroxydecanoic acid | at pH 8.0 and 25°C | Stenotrophomonas maltophilia | |
9.6 | - |
syn-2-octyl-3-hydroxydodecanoic acid | at pH 8.0 and 25°C | Stenotrophomonas maltophilia | |
47.9 | - |
syn-2-decyl-3-hydroxytetradecanoic acid | at pH 8.0 and 25°C | Stenotrophomonas maltophilia |