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Literature summary for 1.1.1.404 extracted from

  • Yadid, I.; Rudolph, J.; Hlouchova, K.; Copley, S.D.
    Sequestration of a highly reactive intermediate in an evolving pathway for degradation of pentachlorophenol (2013), Proc. Natl. Acad. Sci. USA, 110, E2182-E2190.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0115
-
NADH pH 7.6, 22°C Sphingobium chlorophenolicum
0.0176
-
cytochrome c pH 7.6, 22°C Sphingobium chlorophenolicum
0.0383
-
2,6-dichloroindophenol pH 7.6, 22°C Sphingobium chlorophenolicum
1.21
-
NADPH pH 7.6, 22°C Sphingobium chlorophenolicum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
36000
-
-
Sphingobium chlorophenolicum
100000
-
gel fitlration Sphingobium chlorophenolicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+ Sphingobium chlorophenolicum
-
2,3,5,6-tetrachlorohydroquinone + NAD+
-
?
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+ Sphingobium chlorophenolicum RA2
-
2,3,5,6-tetrachlorohydroquinone + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Sphingobium chlorophenolicum M9WIZ7
-
-
Sphingobium chlorophenolicum M9WLE1
-
-
Sphingobium chlorophenolicum RA2 M9WIZ7
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant protein, golden-brown enzyme with a spectrum consistent with the presence of both a flavin and an iron-sulfur cluster Sphingobium chlorophenolicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+
-
Sphingobium chlorophenolicum 2,3,5,6-tetrachlorohydroquinone + NAD+
-
?
2,3,5,6-tetrachloro-1,4-benzoquinone + NADH + H+
-
Sphingobium chlorophenolicum RA2 2,3,5,6-tetrachlorohydroquinone + NAD+
-
?
2,6-dichloroindophenol + NADH + H+
-
Sphingobium chlorophenolicum reduced 2,6-dichloroindophenol + NAD+
-
?
2,6-dichloroindophenol + NADH + H+
-
Sphingobium chlorophenolicum RA2 reduced 2,6-dichloroindophenol + NAD+
-
?
cytochrome c + NADPH + H+
-
Sphingobium chlorophenolicum reduced cytochrome c + NADP+
-
?
cytochrome c + NADPH + H+
-
Sphingobium chlorophenolicum RA2 reduced cytochrome c + NADP+
-
?

Subunits

Subunits Comment Organism
homotrimer 3 * 36000, calculated Sphingobium chlorophenolicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.7
-
NADPH pH 7.6, 22°C Sphingobium chlorophenolicum
9.1
-
cytochrome c pH 7.6, 22°C Sphingobium chlorophenolicum
14.1
-
2,6-dichloroindophenol pH 7.6, 22°C Sphingobium chlorophenolicum
20.1
-
NADH pH 7.6, 22°C Sphingobium chlorophenolicum

Cofactor

Cofactor Comment Organism Structure
FMN
-
Sphingobium chlorophenolicum
NADH
-
Sphingobium chlorophenolicum
NADPH poor activity with NADPH Sphingobium chlorophenolicum

General Information

General Information Comment Organism
physiological function tetrachlorohydroquinone reductase PcpD catalyzes the reduction of tetrachlorohydroquinone to tetrachlorohydroquinone. In the presence of PcpD, tetrachlorohydroquinone formed by pentachlorophenol hydroxylase PcpB is sequestered until it is reduced to the less toxic tetrachlorohydroquinone, protecting the bacterium from the toxic effects of tetrachlorohydroquinone and maintaining flux through the pathway. Deletion of pcpD results in increased sensitivity to phenols that are degraded via a benzoquinone intermediate Sphingobium chlorophenolicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.1
-
NADPH pH 7.6, 22°C Sphingobium chlorophenolicum
368
-
2,6-dichloroindophenol pH 7.6, 22°C Sphingobium chlorophenolicum
510
-
cytochrome c pH 7.6, 22°C Sphingobium chlorophenolicum
1740
-
NADH pH 7.6, 22°C Sphingobium chlorophenolicum
12000
-
2,3,5,6-tetrachloro-1,4-benzoquinone pH 7.6, 22°C Sphingobium chlorophenolicum