Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta(DE3) cells | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
S419A | the variant presents 72.3% of the wild type activity | Zea mays |
S419E | the variant presents 8.7% of the wild type activity. The mutation dramatically decreases the affinity for the cofactor, as saturation is not observed even at NADP+ concentrations as high as 5 mM | Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0123 | - |
NADP+ | wild type enzyme, at pH 8.0 and 25°C | Zea mays | |
0.1595 | - |
NADP+ | mutant enzyme S419A, at pH 8.0 and 25°C | Zea mays | |
0.19 | - |
(S)-malate | wild type enzyme, at pH 8.0 and 25°C | Zea mays | |
0.47 | - |
(S)-malate | mutant enzyme S419A, at pH 8.0 and 25°C | Zea mays | |
2.68 | - |
(S)-malate | mutant enzyme S419A, at pH 8.0 and 25°C | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chloroplast | - |
Zea mays | 9507 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM used in assay conditions | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Zea mays | - |
pyruvate + CO2 + NADPH + H+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | Zea mays | - |
(S)-malate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | P16243 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylation of the enzyme at Ser419 drastically decreases its activity. Phosphorylation of isoform C4-NADP-ME at Ser419 during the first hours in the light is a cellular mechanism that fine tunes the enzymatic activity to coordinate the carbon concentration mechanism with the CO2 fixation rate, probably to avoid CO2 leakiness from bundle sheath cells | Zea mays |
Purification (Comment) | Organism |
---|---|
Ni-NTA agarose column chromatography | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Zea mays | pyruvate + CO2 + NADPH + H+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | - |
Zea mays | (S)-malate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 63400, calculated from amino acid sequence | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
C4-NADP-ME | isoform | Zea mays |
NADP-malic enzyme | - |
Zea mays |
NADP-ME | - |
Zea mays |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.1 | - |
NADP+ | mutant enzyme S419A, at pH 8.0 and 25°C | Zea mays | |
8.3 | - |
NADP+ | mutant enzyme S419A, at pH 8.0 and 25°C | Zea mays | |
12.2 | - |
NADP+ | wild type enzyme, at pH 8.0 and 25°C | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Zea mays | |
NADPH | - |
Zea mays |