Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Rhodococcus jostii |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
sesamol | - |
Rhodococcus fascians | |
sesamol | - |
Rhodococcus jostii | |
sesamol | - |
Rhodococcus opacus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Rhodococcus opacus | 5829 | - |
cytosol | - |
Rhodococcus fascians | 5829 | - |
cytosol | - |
Rhodococcus jostii | 5829 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 10 mM used in assay conditions | Rhodococcus opacus | |
Mg2+ | 10 mM used in assay conditions | Rhodococcus fascians | |
Mg2+ | 10 mM used in assay conditions | Rhodococcus jostii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Rhodococcus opacus | - |
pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | Rhodococcus fascians | - |
pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | Rhodococcus jostii | - |
pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | Rhodococcus opacus PD630 | - |
pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | Rhodococcus fascians F7 | - |
pyruvate + CO2 + NADPH + H+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | Rhodococcus opacus | - |
(S)-malate + NADP+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | Rhodococcus fascians | - |
(S)-malate + NADP+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | Rhodococcus jostii | - |
(S)-malate + NADP+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | Rhodococcus opacus PD630 | - |
(S)-malate + NADP+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | Rhodococcus fascians F7 | - |
(S)-malate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodococcus fascians | - |
- |
- |
Rhodococcus fascians F7 | - |
- |
- |
Rhodococcus jostii | - |
- |
- |
Rhodococcus opacus | - |
- |
- |
Rhodococcus opacus PD630 | - |
- |
- |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.0019 | - |
in the presence of 0.1 g/l ammonium chloride, at pH 7.5 and 30°C | Rhodococcus fascians |
0.0021 | - |
in the presence of 1.0 g/l ammonium chloride, at pH 7.5 and 30°C | Rhodococcus fascians |
0.0023 | - |
in the absence of ammonium chloride, at pH 7.5 and 30°C | Rhodococcus fascians |
0.0134 | - |
in the presence of 1.0 g/l ammonium chloride and 1.5 mM sesamol, at pH 7.5 and 30°C | Rhodococcus jostii |
0.0217 | - |
in the presence of 0.1 g/l ammonium chloride and 1.5 mM sesamol, at pH 7.5 and 30°C | Rhodococcus jostii |
0.0225 | - |
in the absence of ammonium chloride and in the presence of 1.5 mM sesamol, at pH 7.5 and 30°C | Rhodococcus jostii |
0.0234 | - |
in the presence of 1.0 g/l ammonium chloride, at pH 7.5 and 30°C | Rhodococcus jostii |
0.0342 | - |
in the absence of ammonium chloride, at pH 7.5 and 30°C | Rhodococcus jostii |
0.0435 | - |
in the presence of 0.1 g/l ammonium chloride, at pH 7.5 and 30°C | Rhodococcus jostii |
0.059 | - |
in the presence of 1.0 g/l ammonium chloride, at pH 7.5 and 30°C | Rhodococcus opacus |
0.1343 | - |
in the absence of ammonium chloride, at pH 7.5 and 30°C | Rhodococcus opacus |
0.1533 | - |
in the presence of 0.1 g/l ammonium chloride, at pH 7.5 and 30°C | Rhodococcus opacus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Rhodococcus opacus | pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | - |
Rhodococcus fascians | pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | - |
Rhodococcus jostii | pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | - |
Rhodococcus opacus PD630 | pyruvate + CO2 + NADPH + H+ | - |
r | |
(S)-malate + NADP+ | - |
Rhodococcus fascians F7 | pyruvate + CO2 + NADPH + H+ | - |
r | |
additional information | no activity with NAD+ | Rhodococcus opacus | ? | - |
- |
|
additional information | no activity with NAD+ | Rhodococcus fascians | ? | - |
- |
|
additional information | no activity with NAD+ | Rhodococcus jostii | ? | - |
- |
|
additional information | no activity with NAD+ | Rhodococcus opacus PD630 | ? | - |
- |
|
additional information | no activity with NAD+ | Rhodococcus fascians F7 | ? | - |
- |
|
pyruvate + CO2 + NADPH + H+ | - |
Rhodococcus opacus | (S)-malate + NADP+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | - |
Rhodococcus fascians | (S)-malate + NADP+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | - |
Rhodococcus jostii | (S)-malate + NADP+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | - |
Rhodococcus opacus PD630 | (S)-malate + NADP+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | - |
Rhodococcus fascians F7 | (S)-malate + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
NADP+-dependent malic enzyme | - |
Rhodococcus opacus |
NADP+-dependent malic enzyme | - |
Rhodococcus fascians |
NADP+-dependent malic enzyme | - |
Rhodococcus jostii |
NADP+-ME | - |
Rhodococcus opacus |
NADP+-ME | - |
Rhodococcus fascians |
NADP+-ME | - |
Rhodococcus jostii |
RHA1_RS44255 | - |
Rhodococcus jostii |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | the enzyme prefers NADP+ as a coenzyme | Rhodococcus opacus | |
NADP+ | the enzyme prefers NADP+ as a coenzyme | Rhodococcus fascians | |
NADP+ | the enzyme prefers NADP+ as a coenzyme | Rhodococcus jostii |
Organism | Comment | Expression |
---|---|---|
Rhodococcus jostii | total enzyme activity increases by 1.8fold during cultivation under nitrogen-limiting conditions | up |
Rhodococcus opacus | total enzyme activity increases by 2.6fold during cultivation under nitrogen-limiting conditions | up |