Literature summary for 1.1.1.40 extracted from

Rozova, O.N.; Khmelenina, V.N.; Mustakhimov, I.I.; But, S.Y.; Trotsenko, Y.A.
Properties of malic enzyme from the aerobic methanotroph Methylosinus trichosporium (2019), Biochemistry (Moscow), 84, 390-397 .

Search for more literature for 1.1.1.40

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Methylosinus trichosporium

Inhibitors

Inhibitors	Comment	Organism	Structure
acetyl-CoA	acetyl-CoA (0.1 mM) exerts the greatest inhibitory effect leading to a residual activity of 24%	Methylosinus trichosporium
ATP	ATP at 2 mM inhibits the enzyme by 50%, but the activity is completely recovered if Mg2+ concentration increases to 5 mM	Methylosinus trichosporium
Cd2+	in the presence of Mn2+, Cd2+ ions almost completely inhibit the enzyme activity (5.9% residual activity)	Methylosinus trichosporium
diphosphate	diphosphate at 2 mM inhibits the enzyme by 50%, but the activity is completely recovered if Mg2+ concentration increases to 5 mM	Methylosinus trichosporium
Hydroxypyruvate	hydroxypyruvate at 1 mM inhibits the enzyme activity by 45%	Methylosinus trichosporium
NaCl	in the presence of 50 mM KCl, 50 mM NaCl inhibits the enzyme activity by 40%	Methylosinus trichosporium
Sn2+	1 mM Sn2+ ions reduce the enzyme activity by 31%	Methylosinus trichosporium

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.047	-	NADPH	at pH 7.0 and 30°C	Methylosinus trichosporium
0.064	-	NADP+	at pH 7.0 and 30°C	Methylosinus trichosporium
2.7	-	(S)-malate	at pH 7.0 and 30°C	Methylosinus trichosporium
6	-	pyruvate	at pH 7.0 and 30°C	Methylosinus trichosporium

Metals/Ions

Metals/Ions	Comment	Organism	Structure
K+	100% activity in the presence of K+ and Mg2+ (1 mM each), 123.3% activity in the presence of K+ plus Mn2+ (1 mM each)	Methylosinus trichosporium
Mg2+	100% activity in the presence of K+ plus Mg2+ (1 mM each), 115.2% activity in the presence of K+ plus NH4+ (1 mM each)	Methylosinus trichosporium
Mn2+	123.3% activity in the presence of K+ plus Mn2+ (1 mM each)	Methylosinus trichosporium
NH4+	115.2% activity in the presence of K+ plus NH4+ (1 mM each)	Methylosinus trichosporium

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
480000	-	gel filtration	Methylosinus trichosporium

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(S)-malate + NADP+	Methylosinus trichosporium	the enzyme demonstrates 4.7fold higher activity in the direction of malate decarboxylation compared with pyruvate carboxylation	pyruvate + CO2 + NADPH + H+	-	?
pyruvate + CO2 + NADPH + H+	Methylosinus trichosporium	-	(S)-malate + NADP+	-	r

Organism

Organism	UniProt	Comment	Textmining
Methylosinus trichosporium	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-NTA agarose column chromatography	Methylosinus trichosporium

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(S)-malate + NADP+	the enzyme demonstrates 4.7fold higher activity in the direction of malate decarboxylation compared with pyruvate carboxylation	Methylosinus trichosporium	pyruvate + CO2 + NADPH + H+	-	?
additional information	the enzyme does not use NAD+ and does not show activity of oxaloacetate decarboxylation. NaHCO3 does not serve as a CO2 donor for carboxylation of pyruvate	Methylosinus trichosporium	?	-	-
pyruvate + CO2 + NADPH + H+	-	Methylosinus trichosporium	(S)-malate + NADP+	-	r

Subunits

Subunits	Comment	Organism
homohexamer	6 * 80000, SDS-PAGE	Methylosinus trichosporium
homohexamer	6 * 81500, calculated from amino acid sequence	Methylosinus trichosporium

Synonyms

Synonyms	Comment	Organism
NADP+-ME	-	Methylosinus trichosporium

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Methylosinus trichosporium

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
20	70	the enzyme is active in wide ranges of temperatures (20-70°C)	Methylosinus trichosporium

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
30	60	the enzyme activity is not decreased after incubating the protein for 3 h at 30-40°C, but decreased by half after heating for 1 h at 50°C, and 80% activity is lost after 5 min heating at 60°C	Methylosinus trichosporium

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.067	-	pyruvate	at pH 7.0 and 30°C	Methylosinus trichosporium
0.3	-	(S)-malate	at pH 7.0 and 30°C	Methylosinus trichosporium

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	-	Methylosinus trichosporium

pH Range

pH Minimum	pH Maximum	Comment	Organism
6	9	the enzyme is active in wide ranges of pH (pH 6.0-9.0)	Methylosinus trichosporium

IC50 Value

IC50 Value	IC50 Value Maximum	Comment	Organism	Inhibitor	Structure
2	-	at pH 7.0 and 30°C	Methylosinus trichosporium	ATP
2	-	at pH 7.0 and 30°C	Methylosinus trichosporium	diphosphate

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
0.01	-	pyruvate	at pH 7.0 and 30°C	Methylosinus trichosporium
0.117	-	(S)-malate	at pH 7.0 and 30°C	Methylosinus trichosporium

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Release 2023.1 (January 2023)