Inhibitors | Comment | Organism | Structure |
---|---|---|---|
peroxynitrite | peroxynitrite inhibits cytosolic NADP-ME2 activity due to tyrosine nitration at Tyr-73 to 3-nitrotyrosine | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Arabidopsis thaliana | 5829 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | Arabidopsis thaliana | - |
pyruvate + CO2 + NADPH + H+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | Arabidopsis thaliana | - |
(S)-malate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | - |
ecotype Columbia | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
nitration | Tyr-73 of the enzyme is exclusively nitrated to 3-nitrotyrosine by peroxynitrite | Arabidopsis thaliana |
Purification (Comment) | Organism |
---|---|
LY-TRAP column chromatography | Arabidopsis thaliana |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Arabidopsis thaliana | - |
seedling | - |
Arabidopsis thaliana | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NADP+ | - |
Arabidopsis thaliana | pyruvate + CO2 + NADPH + H+ | - |
r | |
pyruvate + CO2 + NADPH + H+ | - |
Arabidopsis thaliana | (S)-malate + NADP+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 84300, LY-tagged enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
NADP-malate dehydrogenase | - |
Arabidopsis thaliana |
NADP-malic enzyme | - |
Arabidopsis thaliana |
NADP-ME2 | isoform | Arabidopsis thaliana |
Organism | Comment | Expression |
---|---|---|
Arabidopsis thaliana | 25% diminished cytosolic enzyme activity is found in Arabidopsis thaliana seedlings exposed to short-term low temperature (4°C for 48 h) | down |