Cloned (Comment) | Organism |
---|---|
gene bdhA, recombinant expression of N-terminally StrepII-tagged enzyme in Escherichia coli strain BL21(DE3) | Alkalihalobacillus clausii |
Protein Variants | Comment | Organism |
---|---|---|
additional information | the biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated | Alkalihalobacillus clausii |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | 1 mM Mn2+ increases BcBDH's activity in biotransformations | Alkalihalobacillus clausii |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3R)-butane-2,3-diol + NAD+ | Alkalihalobacillus clausii | - |
(3R)-acetoin + NADH + H+ | - |
r | |
(2R,3R)-butane-2,3-diol + NAD+ | Alkalihalobacillus clausii DSM 8716T | - |
(3R)-acetoin + NADH + H+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Alkalihalobacillus clausii | A0A223LRZ7 | - |
- |
Alkalihalobacillus clausii DSM 8716T | A0A223LRZ7 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally StrepII-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography | Alkalihalobacillus clausii |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3R)-butane-2,3-diol + NAD+ | - |
Alkalihalobacillus clausii | (3R)-acetoin + NADH + H+ | - |
r | |
(2R,3R)-butane-2,3-diol + NAD+ | - |
Alkalihalobacillus clausii DSM 8716T | (3R)-acetoin + NADH + H+ | - |
r | |
2,3-hexanedione + NADH + H+ | small amounts of (2R)-2-hydroxy-3-hexanone are produced as by-product | Alkalihalobacillus clausii | (3R)-3-hydroxy-2-hexanone + NAD+ | - |
r | |
2,3-hexanedione + NADH + H+ | small amounts of (2R)-2-hydroxy-3-hexanone are produced as by-product | Alkalihalobacillus clausii DSM 8716T | (3R)-3-hydroxy-2-hexanone + NAD+ | - |
r | |
2,3-pentanedione + NADH + H+ | small amounts of 2,3-pentanediol are produced as by-product | Alkalihalobacillus clausii | 3-hydroxy-2-pentanone + NAD+ | - |
r | |
2,3-pentanedione + NADH + H+ | small amounts of 2,3-pentanediol are produced as by-product | Alkalihalobacillus clausii DSM 8716T | 3-hydroxy-2-pentanone + NAD+ | - |
r | |
3,4-hexanedione + 2 NADH + 2 H+ | - |
Alkalihalobacillus clausii | (3R,4R)-3,4-hexanediol + 2 NAD+ | - |
r | |
5-methyl-2,3-hexanedione + NADH + H+ | small amounts of 5-methyl-2-hydroxy-3-hexanone and traces of (3S)-5-methyl-3-hydroxy-2-hexanone are produced as by-products | Alkalihalobacillus clausii | (3R)-5-methyl-3-hydroxy-2-hexanone + NAD+ | - |
r | |
5-methyl-2,3-hexanedione + NADH + H+ | small amounts of 5-methyl-2-hydroxy-3-hexanone and traces of (3S)-5-methyl-3-hydroxy-2-hexanone are produced as by-products | Alkalihalobacillus clausii DSM 8716T | (3R)-5-methyl-3-hydroxy-2-hexanone + NAD+ | - |
r | |
additional information | substrate specificity analysis, overview. Enzyme BcBDH catalyzes the selective asymmetric reduction of prochiral 1,2-diketones to the corresponding HK and, in some cases, the reduction of the same to the corresponding 1,2-diols. Aliphatic diketones, like 2,3-pentanedione, 2,3-hexanedione, 5-methyl-2,3-hexanedione, 3,4-hexanedione, and 2,3-heptanedione are well transformed. In addition, surprisingly alkyl phenyl dicarbonyls, like 2-hydroxy-1-phenylpropan-1-one and phenylglyoxal are accepted, whereas their derivatives with two phenyl groups are not substrates. The biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated. Stereoselectivity and conversion is analyzed by carrying out the reduction reaction of selected diketones and alpha-hydroxyketones | Alkalihalobacillus clausii | ? | - |
- |
|
additional information | substrate specificity analysis, overview. Enzyme BcBDH catalyzes the selective asymmetric reduction of prochiral 1,2-diketones to the corresponding HK and, in some cases, the reduction of the same to the corresponding 1,2-diols. Aliphatic diketones, like 2,3-pentanedione, 2,3-hexanedione, 5-methyl-2,3-hexanedione, 3,4-hexanedione, and 2,3-heptanedione are well transformed. In addition, surprisingly alkyl phenyl dicarbonyls, like 2-hydroxy-1-phenylpropan-1-one and phenylglyoxal are accepted, whereas their derivatives with two phenyl groups are not substrates. The biocatalytic reduction of 5-methyl-2,3-hexanedione to mainly 5-methyl-3-hydroxy-2-hexanone with only small amounts of 5-methyl-2-hydroxy-3-hexanone within an enzyme membrane reactor is demonstrated. Stereoselectivity and conversion is analyzed by carrying out the reduction reaction of selected diketones and alpha-hydroxyketones | Alkalihalobacillus clausii DSM 8716T | ? | - |
- |
Synonyms | Comment | Organism |
---|---|---|
(R,R)-butane-2,3-diol dehydrogenase | - |
Alkalihalobacillus clausii |
BcBDH | - |
Alkalihalobacillus clausii |
BdhA | - |
Alkalihalobacillus clausii |
butanediol dehydrogenase | - |
Alkalihalobacillus clausii |
R-selective 2,3-butanediol dehydrogenase | - |
Alkalihalobacillus clausii |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Alkalihalobacillus clausii |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.8 | - |
assay at | Alkalihalobacillus clausii |
General Information | Comment | Organism |
---|---|---|
evolution | the R-selective 2,3-butanediol dehydrogenase from Bacillus clausii strain DSM 8716 (BcBDH) belongs to the metal-dependent medium chain dehydrogenases/reductases family (MDR) | Alkalihalobacillus clausii |