Literature summary for 1.1.1.4 extracted from

Li, P.; Guo, X.; Shi, T.; Hu, Z.; Chen, Y.; Du, L.; Xiao, D.
Reducing diacetyl production of wine by overexpressing BDH1 and BDH2 in Saccharomyces uvarum (2017), J. Ind. Microbiol. Biotechnol., 44, 1541-1550 .

Cloned(Commentary)

Cloned (Comment)	Organism
gene BDH1, construction of plasmid B1ZF-loxP-KanMX-loxPPGK1p-BDH1-PGK1t-B1ZR in Escherichia coli strain DH5alpha, and recombinant overexpression in Saccharomyces uvarum strain WY1 resulting in strain WY1-1, overexpression of gene BDH2 from Saccharomyces uvarum (WY1-2), method overview. BDH1 and BDH2 overexpression in one strain, named WY1-12, is constructed on the basis of WY1-2, that is, the strain WY1-2 is transformed into plasmid Yep-KPB1 after excising KanMX, real-time PCR expression analysis	Saccharomyces uvarum

Cloned (Comment)

Organism

gene BDH1, construction of plasmid B1ZF-loxP-KanMX-loxPPGK1p-BDH1-PGK1t-B1ZR in Escherichia coli strain DH5alpha, and recombinant overexpression in Saccharomyces uvarum strain WY1 resulting in strain WY1-1, overexpression of gene BDH2 from Saccharomyces uvarum (WY1-2), method overview. BDH1 and BDH2 overexpression in one strain, named WY1-12, is constructed on the basis of WY1-2, that is, the strain WY1-2 is transformed into plasmid Yep-KPB1 after excising KanMX, real-time PCR expression analysis

Saccharomyces uvarum

Protein Variants

Protein Variants	Comment	Organism
additional information	construction of a an BDH1 deletion strain, WY1DELTA1, which shows 1.37fold increased diacetyl production	Saccharomyces uvarum

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
(R)-acetoin + NADH + H+	Saccharomyces uvarum	-	(R,R)-butane-2,3-diol + NAD+	-	r
(R)-acetoin + NADH + H+	Saccharomyces uvarum WY1	-	(R,R)-butane-2,3-diol + NAD+	-	r

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces uvarum	-	-	-
Saccharomyces uvarum WY1	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(R)-acetoin + NADH + H+	-	Saccharomyces uvarum	(R,R)-butane-2,3-diol + NAD+	-	r
(R)-acetoin + NADH + H+	-	Saccharomyces uvarum WY1	(R,R)-butane-2,3-diol + NAD+	-	r

Synonyms

Synonyms	Comment	Organism
2,3-butanediol dehydrogenase	-	Saccharomyces uvarum
BDH1	-	Saccharomyces uvarum
Bdh1p	-	Saccharomyces uvarum

Cofactor

Cofactor	Comment	Organism	Structure
NAD+	-	Saccharomyces uvarum
NADH	-	Saccharomyces uvarum

Expression

Organism	Comment	Expression
Saccharomyces uvarum	BDH2 is a gene adjacent to BDH1, and these genes are regulated reciprocally	additional information

General Information

General Information	Comment	Organism
metabolism	BDH2 is a gene adjacent to BDH1, and these genes are regulated reciprocally. BDH2 is only responsible for converting diacetyl into acetoin, but not for the metabolic pathway of acetoin to 2,3-butanediol in Saccharomyces uvarum	Saccharomyces uvarum
physiological function	the enzyme is involved in formation of diacetyl in wine	Saccharomyces uvarum