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Literature summary for 1.1.1.4 extracted from

  • Kang, I.Y.; Park, J.M.; Hong, W.K.; Kim, Y.S.; Jung, Y.R.; Kim, S.B.; Heo, S.Y.; Lee, S.M.; Kang, J.Y.; Oh, B.R.; Kim, D.H.; Seo, J.W.; Kim, C.H.
    Enhanced production of 2,3-butanediol by a genetically engineered Bacillus sp. BRC1 using a hydrolysate of empty palm fruit bunches (2015), Bioprocess Biosyst. Eng., 38, 299-305.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.12
-
(R)-acetoin at pH 6.0 and 50┬░C Bacillus sp. (in: Bacteria)
0.13
-
diacetyl at pH 6.0 and 50┬░C Bacillus sp. (in: Bacteria)
6.01
-
(R,R)-butane-2,3-diol at pH 9.0 and 50┬░C Bacillus sp. (in: Bacteria)
6.38
-
meso-butane-2,3-diol at pH 9.0 and 50┬░C Bacillus sp. (in: Bacteria)

Metals/Ions

Metals/Ions Comment Organism Structure
Zn2+ contains zinc Bacillus sp. (in: Bacteria)

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R,R)-butane-2,3-diol + NAD+ Bacillus sp. (in: Bacteria) preferred substrate, 100% activity (R)-acetoin + NADH + H+
-
?
(R,R)-butane-2,3-diol + NAD+ Bacillus sp. (in: Bacteria) BRC1 preferred substrate, 100% activity (R)-acetoin + NADH + H+
-
?

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria) A0A075BZ18
-
-
Bacillus sp. (in: Bacteria) BRC1 A0A075BZ18
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R,R)-butane-2,3-diol + NAD+ preferred substrate, 100% activity Bacillus sp. (in: Bacteria) (R)-acetoin + NADH + H+
-
?
(R,R)-butane-2,3-diol + NAD+ preferred substrate, 100% activity Bacillus sp. (in: Bacteria) BRC1 (R)-acetoin + NADH + H+
-
?
butane-1,2-diol + NAD+ 76.6% activity compared to (R,R)-butane-2,3-diol Bacillus sp. (in: Bacteria) ?
-
?
butane-1,2-diol + NAD+ 76.6% activity compared to (R,R)-butane-2,3-diol Bacillus sp. (in: Bacteria) BRC1 ?
-
?
butane-1,4-diol + NAD+ 2.9% activity compared to (R,R)-butane-2,3-diol Bacillus sp. (in: Bacteria) ?
-
?
diacetyl + NADH + H+ 88.2% activity compared to acetoin Bacillus sp. (in: Bacteria) acetoin + NAD+
-
?
diacetyl + NADH + H+ 88.2% activity compared to acetoin Bacillus sp. (in: Bacteria) BRC1 acetoin + NAD+
-
?
isobutanol + NAD+ 0.4% activity compared to (R,R)-butane-2,3-diol Bacillus sp. (in: Bacteria) ?
-
?
meso-butane-2,3-diol + NAD+ 93.6% activity compared to (R,R)-butane-2,3-diol Bacillus sp. (in: Bacteria) acetoin + NADH + H+
-
?
meso-butane-2,3-diol + NAD+ 93.6% activity compared to (R,R)-butane-2,3-diol Bacillus sp. (in: Bacteria) BRC1 acetoin + NADH + H+
-
?
additional information no activity with (S,S)-butane-2,3-diol Bacillus sp. (in: Bacteria) ?
-
?
additional information no activity with (S,S)-butane-2,3-diol Bacillus sp. (in: Bacteria) BRC1 ?
-
?
propane-1,2-diol + NAD+ 58.6% activity compared to (R,R)-butane-2,3-diol Bacillus sp. (in: Bacteria) ?
-
?
propane-1,3-diol + NAD+ 1.4% activity compared to (R,R)-butane-2,3-diol Bacillus sp. (in: Bacteria) ?
-
?

Synonyms

Synonyms Comment Organism
BDH
-
Bacillus sp. (in: Bacteria)
D-2,3-BD dehydrogenase
-
Bacillus sp. (in: Bacteria)

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
50
-
-
Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
reductive reaction Bacillus sp. (in: Bacteria)
9
-
oxidative reaction Bacillus sp. (in: Bacteria)

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Bacillus sp. (in: Bacteria)