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Literature summary for 1.1.1.4 extracted from

  • Koepke, M.; Gerth, M.L.; Maddock, D.J.; Mueller, A.P.; Liew, F.; Simpson, S.D.; Patrick, W.M.
    Reconstruction of an acetogenic 2,3-butanediol pathway involving a novel NADPH-dependent primary-secondary alcohol dehydrogenase (2014), Appl. Environ. Microbiol., 80, 3394-3403.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.6
-
acetone pH 7.5, 25°C Clostridium autoethanogenum
1.2
-
Butanone pH 7.5, 25°C Clostridium autoethanogenum
5.5
-
acetaldehyde pH 7.5, 25°C Clostridium autoethanogenum
65
-
(2R,3R)-2,3-butanediol pH 7.5, 25°C Clostridium autoethanogenum
71
-
acetoin pH 7.5, 25°C Clostridium autoethanogenum

Organism

Organism UniProt Comment Textmining
Clostridium autoethanogenum F8TEL7
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3R)-2,3-butanediol + NADP+ no detectable activity with (2S,3S)-2,3-butanediol Clostridium autoethanogenum (R)-acetoin + NADPH + H+
-
r
acetaldehyde + NADPH + H+
-
Clostridium autoethanogenum ethanol + NADP+
-
?
acetoin + 2 NADPH + 2 H+
-
Clostridium autoethanogenum (2R,3R)-2,3-butanediol + meso-2,3-butanediol + 2 NADP+
-
r
acetone + NADPH + H+
-
Clostridium autoethanogenum propan-2-ol + NADP+
-
?
butanone + NADPH + H+
-
Clostridium autoethanogenum butan-2-ol + NADP+
-
?
additional information enzyme is a strictly NADPH-dependent primary-secondary alcohol dehydrogenase able to reduce acetoin to 2,3-butanediol. The enzyme accepts a range of 2-, 3-, and 4-carbon substrates, including the nonphysiological ketones acetone and butanone Clostridium autoethanogenum ?
-
?

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1
-
(2R,3R)-2,3-butanediol pH 7.5, 25°C Clostridium autoethanogenum
44
-
Butanone pH 7.5, 25°C Clostridium autoethanogenum
51.4
-
acetone pH 7.5, 25°C Clostridium autoethanogenum
93
-
acetaldehyde pH 7.5, 25°C Clostridium autoethanogenum
145
-
acetoin pH 7.5, 25°C Clostridium autoethanogenum

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADH Clostridium autoethanogenum
NADPH
-
Clostridium autoethanogenum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.033
-
(2R,3R)-2,3-butanediol pH 7.5, 25°C Clostridium autoethanogenum
2
-
acetoin pH 7.5, 25°C Clostridium autoethanogenum
17
-
acetaldehyde pH 7.5, 25°C Clostridium autoethanogenum
38
-
Butanone pH 7.5, 25°C Clostridium autoethanogenum
86
-
acetone pH 7.5, 25°C Clostridium autoethanogenum