Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparison, subcloning in Escherichia coli strain JM109, expression in Escherichia coli strain BL21 (DE3) | Paenibacillus polymyxa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
(3R,3S)-acetoin | pH 7.0, 30°C | Paenibacillus polymyxa | |
0.32 | - |
NADH | pH 7.0, 30°C | Paenibacillus polymyxa | |
0.84 | - |
acetoin | (S)-acetoin, pH 7.0, 30°C | Paenibacillus polymyxa | |
2.3 | - |
NAD+ | pH 7.0, 30°C | Paenibacillus polymyxa | |
2.73 | - |
meso-butane-2,3-diol | pH 7.0, 30°C | Paenibacillus polymyxa | |
7.67 | - |
(2R,3R)-butane-2,3-diol | pH 7.0, 30°C | Paenibacillus polymyxa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44500 | - |
2 * 44500, SDS-PAGE | Paenibacillus polymyxa |
90000 | - |
gel filtration | Paenibacillus polymyxa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenibacillus polymyxa | - |
isolated from soil | - |
Paenibacillus polymyxa ZJ-9 | - |
isolated from soil | - |
Purification (Comment) | Organism |
---|---|
native enzyme 35fold to homogeneity by fractional ammonium sulfate precipitation, followed by two different steps of anion exchange chromatography | Paenibacillus polymyxa |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | - |
Paenibacillus polymyxa | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
4.65 | - |
purified native enzyme, pH 7.0, 30°C | Paenibacillus polymyxa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(2R,3R)-butane-2,3-diol + NAD+ | - |
Paenibacillus polymyxa | (3R,3S)-acetoin + NADH + H+ | - |
r | |
(2R,3R)-butane-2,3-diol + NAD+ | - |
Paenibacillus polymyxa ZJ-9 | (3R,3S)-acetoin + NADH + H+ | - |
r | |
meso-butane-2,3-diol + NAD+ | - |
Paenibacillus polymyxa | acetoin + NADH + H+ | - |
r | |
meso-butane-2,3-diol + NAD+ | - |
Paenibacillus polymyxa ZJ-9 | acetoin + NADH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 44500, SDS-PAGE | Paenibacillus polymyxa |
Synonyms | Comment | Organism |
---|---|---|
2,3-butanediol dehydrogenase | - |
Paenibacillus polymyxa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Paenibacillus polymyxa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
oxidation reaction | Paenibacillus polymyxa |
8 | - |
reduction reaction | Paenibacillus polymyxa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
additional information | no activity with NADP+ or NADPH as the coenzyme, respectively | Paenibacillus polymyxa | |
NAD+ | - |
Paenibacillus polymyxa | |
NADH | - |
Paenibacillus polymyxa |