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Literature summary for 1.1.1.4 extracted from

  • Gao, J.; Yang, H.H.; Feng, X.H.; Li, S.; Xu, H.
    A 2,3-butanediol dehydrogenase from Paenibacillus polymyxa ZJ-9 for mainly producing R,R-2,3-butanediol: purification, characterization and cloning (2013), J. Basic Microbiol., 53, 733-741.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis, sequence comparison, subcloning in Escherichia coli strain JM109, expression in Escherichia coli strain BL21 (DE3) Paenibacillus polymyxa

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
(3R,3S)-acetoin pH 7.0, 30┬░C Paenibacillus polymyxa
0.32
-
NADH pH 7.0, 30┬░C Paenibacillus polymyxa
0.84
-
acetoin (S)-acetoin, pH 7.0, 30┬░C Paenibacillus polymyxa
2.3
-
NAD+ pH 7.0, 30┬░C Paenibacillus polymyxa
2.73
-
meso-butane-2,3-diol pH 7.0, 30┬░C Paenibacillus polymyxa
7.67
-
(2R,3R)-butane-2,3-diol pH 7.0, 30┬░C Paenibacillus polymyxa

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
44500
-
2 * 44500, SDS-PAGE Paenibacillus polymyxa
90000
-
gel filtration Paenibacillus polymyxa

Organism

Organism UniProt Comment Textmining
Paenibacillus polymyxa
-
isolated from soil
-
Paenibacillus polymyxa ZJ-9
-
isolated from soil
-

Purification (Commentary)

Purification (Comment) Organism
native enzyme 35fold to homogeneity by fractional ammonium sulfate precipitation, followed by two different steps of anion exchange chromatography Paenibacillus polymyxa

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
Paenibacillus polymyxa
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [┬Ámol/min/mg] Specific Activity Maximum [┬Ámol/min/mg] Comment Organism
4.65
-
purified native enzyme, pH 7.0, 30┬░C Paenibacillus polymyxa

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(2R,3R)-butane-2,3-diol + NAD+
-
Paenibacillus polymyxa (3R,3S)-acetoin + NADH + H+
-
r
(2R,3R)-butane-2,3-diol + NAD+
-
Paenibacillus polymyxa ZJ-9 (3R,3S)-acetoin + NADH + H+
-
r
meso-butane-2,3-diol + NAD+
-
Paenibacillus polymyxa acetoin + NADH + H+
-
r
meso-butane-2,3-diol + NAD+
-
Paenibacillus polymyxa ZJ-9 acetoin + NADH + H+
-
r

Subunits

Subunits Comment Organism
dimer 2 * 44500, SDS-PAGE Paenibacillus polymyxa

Synonyms

Synonyms Comment Organism
2,3-butanediol dehydrogenase
-
Paenibacillus polymyxa

Temperature Optimum [┬░C]

Temperature Optimum [┬░C] Temperature Optimum Maximum [┬░C] Comment Organism
30
-
-
Paenibacillus polymyxa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
oxidation reaction Paenibacillus polymyxa
8
-
reduction reaction Paenibacillus polymyxa

Cofactor

Cofactor Comment Organism Structure
additional information no activity with NADP+ or NADPH as the coenzyme, respectively Paenibacillus polymyxa
NAD+
-
Paenibacillus polymyxa
NADH
-
Paenibacillus polymyxa