Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41(DE3) cells | Arabidopsis thaliana |
Crystallization (Comment) | Organism |
---|---|
hanging drop vapor diffusion method, using 0.18 M ammonium sulfate, 0.09 M sodium acetate trihydrate, pH 4.6, 27% (w/v) polyethylene glycol monomethyl ether 2000, and 10% (v/v) glycerol | Arabidopsis thaliana |
General Stability | Organism |
---|---|
the enzyme is sensitive to H2O2 stress. The impact of H2O2 on enzyme activities is probably due to posttranslational modifications | Arabidopsis thaliana |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
NADH | oxidized isoform cytMDH1, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.072 | - |
NADH | reduced isoform cytMDH1, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.143 | - |
oxaloacetate | oxidized isoform cytMDH1, at pH 7.5 and 25°C | Arabidopsis thaliana | |
0.238 | - |
oxaloacetate | reduced isoform cytMDH1, at pH 7.5 and 25°C | Arabidopsis thaliana |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel filtration | Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Arabidopsis thaliana | - |
oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH + H+ | Arabidopsis thaliana | - |
(S)-malate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P93819 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni2+-Sepharose column chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Arabidopsis thaliana | oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Arabidopsis thaliana | (S)-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 37500, calculated from amino acid sequence | Arabidopsis thaliana |
homodimer | 2 * 37000, SDS-PAGE. Thioredoxin-reversible homodimerization of isoform cytMDH1 through Cys330 disulfide formation protects the protein from overoxidation | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
cytMDH1 | - |
Arabidopsis thaliana |
cytosolic malate dehydrogenase | - |
Arabidopsis thaliana |
L-malate:NAD oxidoreductase | - |
Arabidopsis thaliana |
NAD-dependent MDH | - |
Arabidopsis thaliana |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
49.9 | 54.7 | the melting temperature of the reduced isoform cytMDH1 is 54.7°C, while the melting temperature of the oxidized isoform cytMDH1 drops by to 49.9°C | Arabidopsis thaliana |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
343 | - |
NADH | oxidized isoform cytMDH1, at pH 7.5 and 25°C | Arabidopsis thaliana | |
351 | - |
oxaloacetate | oxidized isoform cytMDH1, at pH 7.5 and 25°C | Arabidopsis thaliana | |
608 | - |
oxaloacetate | reduced isoform cytMDH1, at pH 7.5 and 25°C | Arabidopsis thaliana | |
677 | - |
NADH | reduced isoform cytMDH1, at pH 7.5 and 25°C | Arabidopsis thaliana |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Arabidopsis thaliana | |
NADH | - |
Arabidopsis thaliana |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is more efficient in the reductive reaction in the tricarboxylic acid cycle | Arabidopsis thaliana |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2500 | - |
oxaloacetate | oxidized isoform cytMDH1, at pH 7.5 and 25°C | Arabidopsis thaliana | |
2600 | - |
oxaloacetate | reduced isoform cytMDH1, at pH 7.5 and 25°C | Arabidopsis thaliana | |
6900 | - |
NADH | oxidized isoform cytMDH1, at pH 7.5 and 25°C | Arabidopsis thaliana | |
9000 | - |
NADH | reduced isoform cytMDH1, at pH 7.5 and 25°C | Arabidopsis thaliana |