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Literature summary for 1.1.1.37 extracted from

  • Huang, J.; Niazi, A.K.; Young, D.; Rosado, L.A.; Vertommen, D.; Bodra, N.; Abdelgawwad, M.R.; Vignols, F.; Wei, B.; Wahni, K.; Bashandy, T.; Bariat, L.; Van Breusegem, F.; Messens, J.; Reichheld, J.P.
    Self-protection of cytosolic malate dehydrogenase against oxidative stress in Arabidopsis (2018), J. Exp. Bot., 69, 3491-3505 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Arabidopsis thaliana

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging drop vapor diffusion method, using 0.18 M ammonium sulfate, 0.09 M sodium acetate trihydrate, pH 4.6, 27% (w/v) polyethylene glycol monomethyl ether 2000, and 10% (v/v) glycerol Arabidopsis thaliana

General Stability

General Stability Organism
the enzyme is sensitive to H2O2 stress. The impact of H2O2 on enzyme activities is probably due to posttranslational modifications Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.05
-
NADH oxidized isoform cytMDH1, at pH 7.5 and 25°C Arabidopsis thaliana
0.072
-
NADH reduced isoform cytMDH1, at pH 7.5 and 25°C Arabidopsis thaliana
0.143
-
oxaloacetate oxidized isoform cytMDH1, at pH 7.5 and 25°C Arabidopsis thaliana
0.238
-
oxaloacetate reduced isoform cytMDH1, at pH 7.5 and 25°C Arabidopsis thaliana

Localization

Localization Comment Organism GeneOntology No. Textmining

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-malate + NAD+ Arabidopsis thaliana
-
oxaloacetate + NADH + H+
-
r
oxaloacetate + NADH + H+ Arabidopsis thaliana
-
(S)-malate + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana P93819
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-Sepharose column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-malate + NAD+
-
Arabidopsis thaliana oxaloacetate + NADH + H+
-
r
oxaloacetate + NADH + H+
-
Arabidopsis thaliana (S)-malate + NAD+
-
r

Subunits

Subunits Comment Organism
homodimer 2 * 37500, calculated from amino acid sequence Arabidopsis thaliana
homodimer 2 * 37000, SDS-PAGE. Thioredoxin-reversible homodimerization of isoform cytMDH1 through Cys330 disulfide formation protects the protein from overoxidation Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
cytMDH1
-
Arabidopsis thaliana
cytosolic malate dehydrogenase
-
Arabidopsis thaliana
L-malate:NAD oxidoreductase
-
Arabidopsis thaliana
NAD-dependent MDH
-
Arabidopsis thaliana

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
49.9 54.7 the melting temperature of the reduced isoform cytMDH1 is 54.7°C, while the melting temperature of the oxidized isoform cytMDH1 drops by to 49.9°C Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
343
-
NADH oxidized isoform cytMDH1, at pH 7.5 and 25°C Arabidopsis thaliana
351
-
oxaloacetate oxidized isoform cytMDH1, at pH 7.5 and 25°C Arabidopsis thaliana
608
-
oxaloacetate reduced isoform cytMDH1, at pH 7.5 and 25°C Arabidopsis thaliana
677
-
NADH reduced isoform cytMDH1, at pH 7.5 and 25°C Arabidopsis thaliana

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Arabidopsis thaliana
NADH
-
Arabidopsis thaliana

General Information

General Information Comment Organism
metabolism the enzyme is more efficient in the reductive reaction in the tricarboxylic acid cycle Arabidopsis thaliana

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2500
-
oxaloacetate oxidized isoform cytMDH1, at pH 7.5 and 25°C Arabidopsis thaliana
2600
-
oxaloacetate reduced isoform cytMDH1, at pH 7.5 and 25°C Arabidopsis thaliana
6900
-
NADH oxidized isoform cytMDH1, at pH 7.5 and 25°C Arabidopsis thaliana
9000
-
NADH reduced isoform cytMDH1, at pH 7.5 and 25°C Arabidopsis thaliana