Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | - |
Rhodovulum steppense | |
Ca2+ | inhibits isoform MDH1 at millimolar concentrations; inhibits isoform MDH2 at millimolar concentrations; inhibits isoform MDH3 at millimolar concentrations | Rhodovulum steppense | |
citrate | - |
Rhodovulum steppense | |
Mn2+ | slight inhibition of all isoform MDH2; slight inhibition of isoform MDH1; slight inhibition of isoform MDH3 | Rhodovulum steppense | |
oxaloacetate | - |
Rhodovulum steppense |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
oxaloacetate | isoform MDH2, at pH 8.5 and 25°C | Rhodovulum steppense | |
0.052 | - |
oxaloacetate | isoform MDH3, at pH 8.5 and 25°C | Rhodovulum steppense | |
0.06 | - |
NAD+ | isoform MDH1, at pH 8.5 and 25°C | Rhodovulum steppense | |
0.065 | - |
NADH | isoform MDH2, at pH 8.5 and 25°C | Rhodovulum steppense | |
0.075 | - |
NAD+ | isoform MDH3, at pH 8.5 and 25°C | Rhodovulum steppense | |
0.085 | - |
NADH | isoform MDH3, at pH 8.5 and 25°C | Rhodovulum steppense | |
0.11 | - |
NAD+ | isoform MDH2, at pH 8.5 and 25°C | Rhodovulum steppense | |
0.2 | - |
oxaloacetate | isoform MDH1, at pH 8.5 and 25°C | Rhodovulum steppense | |
0.25 | - |
NADH | isoform MDH1, at pH 8.5 and 25°C | Rhodovulum steppense | |
0.95 | - |
(S)-malate | isoform MDH1, at pH 8.5 and 25°C | Rhodovulum steppense | |
1.75 | - |
(S)-malate | isoform MDH3, at pH 8.5 and 25°C | Rhodovulum steppense | |
2.2 | - |
(S)-malate | isoform MDH2, at pH 8.5 and 25°C | Rhodovulum steppense |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | Ca2+ slightly (11-15%) activates isoform MDH1 in the micromolar range | Rhodovulum steppense | |
Ca2+ | Ca2+ slightly (11-15%) activates isoform MDH2 in the micromolar range | Rhodovulum steppense | |
Ca2+ | Ca2+ slightly (11-15%) activates isoform MDH3 in the micromolar range | Rhodovulum steppense | |
Mg2+ | activates isoform MDH1 with Ka value of 5.1 mM | Rhodovulum steppense | |
Mg2+ | activates isoform MDH2 with Ka value of 8.1 mM | Rhodovulum steppense | |
Mg2+ | activates isoform MDH3 with Ka value of 4.7 mM | Rhodovulum steppense |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
isoform MDH3, gel filtration | Rhodovulum steppense |
140000 | - |
isoform MDH2, gel filtration | Rhodovulum steppense |
280000 | - |
isoform MDH1, gel filtration | Rhodovulum steppense |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | Rhodovulum steppense | - |
oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | Rhodovulum steppense A-20s | - |
oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH + H+ | Rhodovulum steppense | - |
(S)-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | Rhodovulum steppense A-20s | - |
(S)-malate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhodovulum steppense | - |
- |
- |
Rhodovulum steppense A-20s | - |
- |
- |
Purification (Comment) | Organism |
---|---|
DEAE cellulose column chromatography and Sephadex G-25 gel filtration | Rhodovulum steppense |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(S)-malate + NAD+ | - |
Rhodovulum steppense | oxaloacetate + NADH + H+ | - |
r | |
(S)-malate + NAD+ | - |
Rhodovulum steppense A-20s | oxaloacetate + NADH + H+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Rhodovulum steppense | (S)-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Rhodovulum steppense A-20s | (S)-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | isoform MDH3, 2 * 35000, SDS-PAGE | Rhodovulum steppense |
homooctamer | isoform MDH1, 8 * 35000, SDS-PAGE | Rhodovulum steppense |
homotetramer | isoform MDH2, 4 * 35000, SDS-PAGE | Rhodovulum steppense |
Synonyms | Comment | Organism |
---|---|---|
Mdh1 | isoform | Rhodovulum steppense |
MDH2 | isoform | Rhodovulum steppense |
Mdh3 | isoform | Rhodovulum steppense |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
temperature optimum of isoform MDH1, with (S)-malate as substrate | Rhodovulum steppense |
40 | - |
temperature optimum of isoform MDH2, with (S)-malate as substrate | Rhodovulum steppense |
45 | - |
temperature optimum of isoform MDH1, with oxaloacetate as substrate | Rhodovulum steppense |
45 | - |
temperature optimum of isoform MDH3, with (S)-malate as substrate | Rhodovulum steppense |
50 | - |
temperature optimum of isoform MDH3, with oxaloacetate as substrate | Rhodovulum steppense |
55 | - |
temperature optimum of isoform MDH2, with oxaloacetate as substrate | Rhodovulum steppense |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
pH optimum of isoform MDH2, with oxaloacetate as substrate | Rhodovulum steppense |
8.5 | - |
pH optimum of isoform MDH3, with oxaloacetate as substrate | Rhodovulum steppense |
9 | - |
pH optimum of isoform MDH2, with (S)-malate as substrate | Rhodovulum steppense |
9.5 | - |
pH optimum of isoform MDH1, with oxaloacetate as substrate | Rhodovulum steppense |
9.5 | - |
pH optimum of isoform MDH3, with (S)-malate as substrate | Rhodovulum steppense |
10.5 | - |
pH optimum of isoform MDH1, with (S)-malate as substrate | Rhodovulum steppense |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Rhodovulum steppense | |
NADH | - |
Rhodovulum steppense |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0019 | - |
oxaloacetate | isoform MDH2, at pH 8.5 and 25°C | Rhodovulum steppense | |
0.0022 | - |
oxaloacetate | isoform MDH3, at pH 8.5 and 25°C | Rhodovulum steppense | |
0.008 | - |
oxaloacetate | isoform MDH1, at pH 8.5 and 25°C | Rhodovulum steppense | |
0.03 | - |
citrate | isoform MDH2, at pH 8.5 and 25°C | Rhodovulum steppense | |
0.06 | - |
citrate | isoform MDH1, at pH 8.5 and 25°C | Rhodovulum steppense | |
0.4 | - |
citrate | isoform MDH3, at pH 8.5 and 25°C | Rhodovulum steppense | |
9 | - |
Ba2+ | isoform MDH2, at pH 8.5 and 25°C | Rhodovulum steppense | |
11 | - |
Ba2+ | isoform MDH1, at pH 8.5 and 25°C | Rhodovulum steppense | |
16 | - |
Ba2+ | isoform MDH3, at pH 8.5 and 25°C | Rhodovulum steppense |