KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.26 | - |
oxaloacetate | at pH 8.0 and 25°C | Zea mays | |
0.33 | - |
NADH | at pH 8.0 and 25°C | Zea mays | |
11.6 | - |
(S)-malate | at pH 8.0 and 25°C | Zea mays | |
129 | - |
NAD+ | at pH 8.0 and 25°C | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
peroxisome | - |
Zea mays | 5777 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
73400 | - |
gel filtration | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH + H+ | Zea mays | - |
(S)-malate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
cultivar Voronezhskaya 76 | - |
Purification (Comment) | Organism |
---|---|
DEAE-Sephacelcolumn chromatography and Sephadex G-25 gel filtration | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zea mays | - |
mesophyll | - |
Zea mays | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3700 | - |
enzyme from homogenate, at pH 8.0 and 25°C | Zea mays |
533000 | - |
after 144fold purification, at pH 8.0 and 25°C | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH + H+ | - |
Zea mays | (S)-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 35500, calculated from amino acid sequence | Zea mays |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Zea mays |
peroxisomal malate dehydrogenase | - |
Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
pH optimum for reverse reaction | Zea mays |
9.5 | - |
pH optimum for forward reaction | Zea mays |