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Literature summary for 1.1.1.37 extracted from
- Isolation, purification, and properties of peroxisomal malate dehydrogenase from maize mesophyll (2018), Appl. Biochem. Microbiol., 54, 320-323 .
No PubMed abstract available
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.26 | - |
oxaloacetate | at pH 8.0 and 25°C | Zea mays |  |
| 0.33 | - |
NADH | at pH 8.0 and 25°C | Zea mays | |
| 11.6 | - |
(S)-malate | at pH 8.0 and 25°C | Zea mays | |
| 129 | - |
NAD+ | at pH 8.0 and 25°C | Zea mays | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| peroxisome | - |
Zea mays | 5777 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 73400 | - |
gel filtration | Zea mays |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxaloacetate + NADH + H+ | Zea mays | - |
(S)-malate + NAD+ | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Zea mays | - |
cultivar Voronezhskaya 76 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| DEAE-Sephacelcolumn chromatography and Sephadex G-25 gel filtration | Zea mays |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Zea mays | - |
| mesophyll | - |
Zea mays | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 3700 | - |
enzyme from homogenate, at pH 8.0 and 25°C | Zea mays |
| 533000 | - |
after 144fold purification, at pH 8.0 and 25°C | Zea mays |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| oxaloacetate + NADH + H+ | - |
Zea mays | (S)-malate + NAD+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 35500, calculated from amino acid sequence | Zea mays |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| MDH | - |
Zea mays |
| peroxisomal malate dehydrogenase | - |
Zea mays |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 9 | - |
pH optimum for reverse reaction | Zea mays |
| 9.5 | - |
pH optimum for forward reaction | Zea mays |
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