Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli (DE3) ArcticExpress cells | Methylorubrum extorquens |
Crystallization (Comment) | Organism |
---|---|
apo enzyme, a binary complex of enzyme and NAD+, and a ternary complex of enzyme and oxaloacetate with ADP-ribose, sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 25-28% (w/v) PEG400, 180-220 mM CaCl2 | Methylorubrum extorquens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0368 | - |
oxaloacetate | at pH 8.1 and 30°C | Methylorubrum extorquens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
137400 | - |
gel filtration | Methylorubrum extorquens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH + H+ | Methylorubrum extorquens | - |
(2S)-malate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylorubrum extorquens | Q84FY8 | - |
- |
Purification (Comment) | Organism |
---|---|
HisTrap column chromatography and Superdex 200 gel filtration | Methylorubrum extorquens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH + H+ | - |
Methylorubrum extorquens | (2S)-malate + NAD+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 34700, His8-tagged enzyme, calculated from amino acid sequence | Methylorubrum extorquens |
Synonyms | Comment | Organism |
---|---|---|
MDH | - |
Methylorubrum extorquens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
460 | - |
oxaloacetate | at pH 8.1 and 30°C | Methylorubrum extorquens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Methylorubrum extorquens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
13000 | - |
oxaloacetate | at pH 8.1 and 30°C | Methylorubrum extorquens |