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Literature summary for 1.1.1.37 extracted from

  • Gonzalez, J.M.; Marti-Arbona, R.; Chen, J.C.H.; Broom-Peltz, B.; Unkefer, C.J.
    Conformational changes on substrate binding revealed by structures of Methylobacterium extorquens malate dehydrogenase (2018), Acta Crystallogr. Sect. F, 74, 610-616 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli (DE3) ArcticExpress cells Methylorubrum extorquens

Crystallization (Commentary)

Crystallization (Comment) Organism
apo enzyme, a binary complex of enzyme and NAD+, and a ternary complex of enzyme and oxaloacetate with ADP-ribose, sitting drop vapor diffusion method, using 0.1 M HEPES pH 7.5, 25-28% (w/v) PEG400, 180-220 mM CaCl2 Methylorubrum extorquens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0368
-
oxaloacetate at pH 8.1 and 30°C Methylorubrum extorquens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
137400
-
gel filtration Methylorubrum extorquens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxaloacetate + NADH + H+ Methylorubrum extorquens
-
(2S)-malate + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Methylorubrum extorquens Q84FY8
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200 gel filtration Methylorubrum extorquens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
oxaloacetate + NADH + H+
-
Methylorubrum extorquens (2S)-malate + NAD+
-
r

Subunits

Subunits Comment Organism
homotetramer 4 * 34700, His8-tagged enzyme, calculated from amino acid sequence Methylorubrum extorquens

Synonyms

Synonyms Comment Organism
MDH
-
Methylorubrum extorquens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
460
-
oxaloacetate at pH 8.1 and 30°C Methylorubrum extorquens

Cofactor

Cofactor Comment Organism Structure
NADH
-
Methylorubrum extorquens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
13000
-
oxaloacetate at pH 8.1 and 30°C Methylorubrum extorquens