Cloned (Comment) | Organism |
---|---|
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression with genes ldh1837 and ldh2043 | Leuconostoc mesenteroides subsp. mesenteroides |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | recombinant L-MDH displays a typical Michaelis-Menten kinetic profile for both substrates | Leuconostoc mesenteroides subsp. mesenteroides | |
29.5 | - |
oxaloacetate | pH 6.0, 25°C | Leuconostoc mesenteroides subsp. mesenteroides | |
98.4 | - |
L-malate | pH 7.0, 25°C | Leuconostoc mesenteroides subsp. mesenteroides |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
oxaloacetate + NADH + H+ | Leuconostoc mesenteroides subsp. mesenteroides | - |
L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | - |
L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 | - |
L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 | - |
L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 | - |
L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 | - |
L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 | - |
L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 | - |
L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 | - |
L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | Leuconostoc mesenteroides subsp. mesenteroides 37Y | - |
L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 | - |
L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 | - |
L-malate + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Leuconostoc mesenteroides subsp. mesenteroides | Q03ZZ4 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides 37Y | Q03ZZ4 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | Q03ZZ4 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 | Q03ZZ4 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 | Q03ZZ4 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 | Q03ZZ4 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 | Q03ZZ4 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 | Q03ZZ4 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 | Q03ZZ4 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 | Q03ZZ4 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 | Q03ZZ4 | - |
- |
Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 | Q03ZZ4 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration | Leuconostoc mesenteroides subsp. mesenteroides |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides 37Y | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 | 2-hydroxybutyrate + NAD+ | - |
r | |
2-oxobutyrate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 | 2-hydroxybutyrate + NAD+ | - |
r | |
L-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides | oxaloacetate + NADH + H+ | - |
r | |
L-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | oxaloacetate + NADH + H+ | - |
r | |
L-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 | oxaloacetate + NADH + H+ | - |
r | |
L-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 | oxaloacetate + NADH + H+ | - |
r | |
L-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 | oxaloacetate + NADH + H+ | - |
r | |
L-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 | oxaloacetate + NADH + H+ | - |
r | |
L-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 | oxaloacetate + NADH + H+ | - |
r | |
L-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 | oxaloacetate + NADH + H+ | - |
r | |
L-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 | oxaloacetate + NADH + H+ | - |
r | |
L-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides 37Y | oxaloacetate + NADH + H+ | - |
r | |
L-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 | oxaloacetate + NADH + H+ | - |
r | |
L-malate + NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 | oxaloacetate + NADH + H+ | - |
r | |
additional information | the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides | ? | - |
- |
|
additional information | the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | ? | - |
- |
|
additional information | the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 | ? | - |
- |
|
additional information | the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 | ? | - |
- |
|
additional information | the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 | ? | - |
- |
|
additional information | the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 | ? | - |
- |
|
additional information | the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 | ? | - |
- |
|
additional information | the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 | ? | - |
- |
|
additional information | the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 | ? | - |
- |
|
additional information | the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides 37Y | ? | - |
- |
|
additional information | the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 | ? | - |
- |
|
additional information | the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate | Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 | ? | - |
- |
|
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides 37Y | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 | L-malate + NAD+ | - |
r | |
oxaloacetate + NADH + H+ | - |
Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 | L-malate + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
L-malate dehydrogenases | - |
Leuconostoc mesenteroides subsp. mesenteroides |
L-MDH | - |
Leuconostoc mesenteroides subsp. mesenteroides |
ldh0076 | - |
Leuconostoc mesenteroides subsp. mesenteroides |
LEUM_0076 | - |
Leuconostoc mesenteroides subsp. mesenteroides |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
recombinant enzyme | Leuconostoc mesenteroides subsp. mesenteroides |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
45 | 20% of maximal activity at 0°C, maximal activity at 30°C. Above the optimum temperature, the L-MDH activity tends to decrease sharply as the temperature increases, inactivation above 45°C | Leuconostoc mesenteroides subsp. mesenteroides |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
oxaloacetate oxidation | Leuconostoc mesenteroides subsp. mesenteroides |
7 | - |
L-malate reduction | Leuconostoc mesenteroides subsp. mesenteroides |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Leuconostoc mesenteroides subsp. mesenteroides | |
NADH | - |
Leuconostoc mesenteroides subsp. mesenteroides |
General Information | Comment | Organism |
---|---|---|
metabolism | in Leuconostoc mesenteroides strain ATCC 8293, which lacks an L-ldh gene, L-lactate is produced through sequential enzymatic conversions from phosphoenolpyruvate to oxaloacetate, then L-malate, and finally L-lactate by phosphoenolpyruvate carboxylase (PEPC, gene ppcA, UniProt ID Q03VI7, LEUM_1694), L-MDH, and malolactic enzyme (MLE, UniProt ID Q03XG6, LEUM_1005), respectively | Leuconostoc mesenteroides subsp. mesenteroides |
additional information | sequence-similarity networks analysis | Leuconostoc mesenteroides subsp. mesenteroides |