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Literature summary for 1.1.1.37 extracted from

  • Kim, K.H.; Jia, X.; Jia, B.; Jeon, C.O.
    Identification and characterization of the L-malate dehydrogenases and L-lactate biosynthetic pathway in Leuconostoc mesenteroides ATCC 8293 (2018), J. Agric. Food Chem., 66, 8086-8093 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3), coexpression with genes ldh1837 and ldh2043 Leuconostoc mesenteroides subsp. mesenteroides

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information recombinant L-MDH displays a typical Michaelis-Menten kinetic profile for both substrates Leuconostoc mesenteroides subsp. mesenteroides
29.5
-
oxaloacetate pH 6.0, 25°C Leuconostoc mesenteroides subsp. mesenteroides
98.4
-
L-malate pH 7.0, 25°C Leuconostoc mesenteroides subsp. mesenteroides

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
oxaloacetate + NADH + H+ Leuconostoc mesenteroides subsp. mesenteroides
-
L-malate + NAD+
-
r
oxaloacetate + NADH + H+ Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293
-
L-malate + NAD+
-
r
oxaloacetate + NADH + H+ Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652
-
L-malate + NAD+
-
r
oxaloacetate + NADH + H+ Leuconostoc mesenteroides subsp. mesenteroides NCDO 523
-
L-malate + NAD+
-
r
oxaloacetate + NADH + H+ Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118
-
L-malate + NAD+
-
r
oxaloacetate + NADH + H+ Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954
-
L-malate + NAD+
-
r
oxaloacetate + NADH + H+ Leuconostoc mesenteroides subsp. mesenteroides JCM 6124
-
L-malate + NAD+
-
r
oxaloacetate + NADH + H+ Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496
-
L-malate + NAD+
-
r
oxaloacetate + NADH + H+ Leuconostoc mesenteroides subsp. mesenteroides DSM 20343
-
L-malate + NAD+
-
r
oxaloacetate + NADH + H+ Leuconostoc mesenteroides subsp. mesenteroides 37Y
-
L-malate + NAD+
-
r
oxaloacetate + NADH + H+ Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023
-
L-malate + NAD+
-
r
oxaloacetate + NADH + H+ Leuconostoc mesenteroides subsp. mesenteroides CCM 1803
-
L-malate + NAD+
-
r

Organism

Organism UniProt Comment Textmining
Leuconostoc mesenteroides subsp. mesenteroides Q03ZZ4
-
-
Leuconostoc mesenteroides subsp. mesenteroides 37Y Q03ZZ4
-
-
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 Q03ZZ4
-
-
Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 Q03ZZ4
-
-
Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 Q03ZZ4
-
-
Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 Q03ZZ4
-
-
Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 Q03ZZ4
-
-
Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 Q03ZZ4
-
-
Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 Q03ZZ4
-
-
Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 Q03ZZ4
-
-
Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 Q03ZZ4
-
-
Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 Q03ZZ4
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Leuconostoc mesenteroides subsp. mesenteroides

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-oxobutyrate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides 2-hydroxybutyrate + NAD+
-
r
2-oxobutyrate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 2-hydroxybutyrate + NAD+
-
r
2-oxobutyrate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 2-hydroxybutyrate + NAD+
-
r
2-oxobutyrate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 2-hydroxybutyrate + NAD+
-
r
2-oxobutyrate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 2-hydroxybutyrate + NAD+
-
r
2-oxobutyrate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 2-hydroxybutyrate + NAD+
-
r
2-oxobutyrate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 2-hydroxybutyrate + NAD+
-
r
2-oxobutyrate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 2-hydroxybutyrate + NAD+
-
r
2-oxobutyrate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 2-hydroxybutyrate + NAD+
-
r
2-oxobutyrate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides 37Y 2-hydroxybutyrate + NAD+
-
r
2-oxobutyrate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 2-hydroxybutyrate + NAD+
-
r
2-oxobutyrate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 2-hydroxybutyrate + NAD+
-
r
L-malate + NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides oxaloacetate + NADH + H+
-
r
L-malate + NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 oxaloacetate + NADH + H+
-
r
L-malate + NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 oxaloacetate + NADH + H+
-
r
L-malate + NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 oxaloacetate + NADH + H+
-
r
L-malate + NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 oxaloacetate + NADH + H+
-
r
L-malate + NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 oxaloacetate + NADH + H+
-
r
L-malate + NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 oxaloacetate + NADH + H+
-
r
L-malate + NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 oxaloacetate + NADH + H+
-
r
L-malate + NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 oxaloacetate + NADH + H+
-
r
L-malate + NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides 37Y oxaloacetate + NADH + H+
-
r
L-malate + NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 oxaloacetate + NADH + H+
-
r
L-malate + NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 oxaloacetate + NADH + H+
-
r
additional information the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate Leuconostoc mesenteroides subsp. mesenteroides ?
-
-
additional information the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 ?
-
-
additional information the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 ?
-
-
additional information the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 ?
-
-
additional information the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 ?
-
-
additional information the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 ?
-
-
additional information the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 ?
-
-
additional information the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 ?
-
-
additional information the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 ?
-
-
additional information the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate Leuconostoc mesenteroides subsp. mesenteroides 37Y ?
-
-
additional information the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 ?
-
-
additional information the recombinant proteins derived from the Escherichia coli cells harboring plasmids pET28a/ldh0076, pET28a/ldh1837, and pET28a/ldh2043 do not show any enzymatic activity toward pyruvate, D-lactate, or L-lactate, indicating that they are not LDHs that catalyze the interconversion of pyruvate and lactate. All three proteins exhibit NADH-oxidation activity toward oxaloacetate and 2-oxobutyrate, producing malate and 2-hydroxybutyrate, respectively. The protein encoded by LEUM_0076 shows clear NAD+-reduction activity toward L-malate, producing oxaloacetate, whereas the other two proteins encoded by LEUM_1837 and LEUM_2043 display NAD+-reduction activity toward D-malate, producing oxaloacetate Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 ?
-
-
oxaloacetate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides L-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides ATCC 8293 L-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides BCRC 11652 L-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides NCDO 523 L-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides NRRL B-1118 L-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides NCTC 12954 L-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides JCM 6124 L-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides NBRC 100496 L-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides DSM 20343 L-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides 37Y L-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides NCIMB 8023 L-malate + NAD+
-
r
oxaloacetate + NADH + H+
-
Leuconostoc mesenteroides subsp. mesenteroides CCM 1803 L-malate + NAD+
-
r

Synonyms

Synonyms Comment Organism
L-malate dehydrogenases
-
Leuconostoc mesenteroides subsp. mesenteroides
L-MDH
-
Leuconostoc mesenteroides subsp. mesenteroides
ldh0076
-
Leuconostoc mesenteroides subsp. mesenteroides
LEUM_0076
-
Leuconostoc mesenteroides subsp. mesenteroides

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
recombinant enzyme Leuconostoc mesenteroides subsp. mesenteroides

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
-
45 20% of maximal activity at 0°C, maximal activity at 30°C. Above the optimum temperature, the L-MDH activity tends to decrease sharply as the temperature increases, inactivation above 45°C Leuconostoc mesenteroides subsp. mesenteroides

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
oxaloacetate oxidation Leuconostoc mesenteroides subsp. mesenteroides
7
-
L-malate reduction Leuconostoc mesenteroides subsp. mesenteroides

Cofactor

Cofactor Comment Organism Structure
NAD+
-
Leuconostoc mesenteroides subsp. mesenteroides
NADH
-
Leuconostoc mesenteroides subsp. mesenteroides

General Information

General Information Comment Organism
metabolism in Leuconostoc mesenteroides strain ATCC 8293, which lacks an L-ldh gene, L-lactate is produced through sequential enzymatic conversions from phosphoenolpyruvate to oxaloacetate, then L-malate, and finally L-lactate by phosphoenolpyruvate carboxylase (PEPC, gene ppcA, UniProt ID Q03VI7, LEUM_1694), L-MDH, and malolactic enzyme (MLE, UniProt ID Q03XG6, LEUM_1005), respectively Leuconostoc mesenteroides subsp. mesenteroides
additional information sequence-similarity networks analysis Leuconostoc mesenteroides subsp. mesenteroides