Cloned (Comment) | Organism |
---|---|
overexpressed in Escherichia coli | Thauera aromatica |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzymew is insensitive to 10 mM EDTA, 1 mM 2,2'-bipyridyl, or 1 mM pyrazole | Thauera aromatica |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.06 | - |
6-hydroxycyclohex-1-ene-1-carbonyl-CoA | pH 7.4, 37°C | Thauera aromatica |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | activity is not increased after adding 2-20 mM of ZnCl2 | Thauera aromatica |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38000 | - |
x * 38000, SDS-PAGE | Thauera aromatica |
78000 | - |
gel filtration | Thauera aromatica |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+ | Thauera aromatica | the enzyme participates in the central benzoyl-CoA degradation pathway of some anaerobic bacteria | 6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+ | - |
ir |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thauera aromatica | O87871 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein | Thauera aromatica |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition | when cells are grown aerobically with benzoate the activity is 20-25fold lower than in cells grown anaerobically with benzoate | Thauera aromatica | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
11.8 | - |
pH 7.4, 37°C, substrate: 6-hydroxycyclohex-1-ene-1-carbonyl-CoA | Thauera aromatica |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+ | the enzyme participates in the central benzoyl-CoA degradation pathway of some anaerobic bacteria | Thauera aromatica | 6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+ | - |
ir | |
6-hydroxycyclohex-1-ene-1-carbonyl-CoA + NAD+ | the enzyme is specific for NAD+, no activity with NADP+. The enzyme is specific for 6-hydroxycyclohex-1-ene-1-carbonyl-CoA, no activity with 2-hydroxycyclohexane-1-carbonyl-CoA | Thauera aromatica | 6-oxocyclohex-1-ene-1-carbonyl-CoA + NADH + H+ | - |
ir |
Subunits | Comment | Organism |
---|---|---|
? | x * 38000, SDS-PAGE | Thauera aromatica |
Synonyms | Comment | Organism |
---|---|---|
HAD | - |
Thauera aromatica |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Thauera aromatica |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
7 | - |
6-hydroxycyclohex-1-ene-1-carbonyl-CoA | pH 7.4, 37°C, calculated as kcat number per subunit | Thauera aromatica |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 7.4 | - |
Thauera aromatica |
7.4 | - |
assay at | Thauera aromatica |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | the enzyme is specific for NAD+, no activity with NADP+ | Thauera aromatica |
Organism | Comment | Expression |
---|---|---|
Thauera aromatica | expression of the enzyme activity is under negative control by oxygen | down |
Thauera aromatica | gene expression is positively controlled by benzoate, an intermediate of the central benzoyl-CoA pathway | up |