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Literature summary for 1.1.1.365 extracted from

  • Cai, X.; Zhang, C.; Ye, J.; Hu, T.; Ye, Z.; Li, H.; Zhang, Y.
    Ectopic expression of FaGalUR leads to ascorbate accumulation with enhanced oxidative stress, cold, and salt tolerance in tomato (2015), Plant Growth Regul., 76, 187-197 .
No PubMed abstract available

Application

Application Comment Organism
nutrition accumulation of high levels of ascorbate using GalUR gene overepression in tomato fruits. Tomato fruits are considered a major dietary source of vitamin C in many countries, because it is consumed regularly and in large quantities. Tomato also serves as a fruit model for other crops species with fleshy berry. Accordingly, it is very important to monitor and to increase the vitamin C content in tomato fruit, for meeting the consumer demand and health requirements for high nutrition, e.g. by overexpressing D-galacturonate reductase Fragaria x ananassa

Cloned(Commentary)

Cloned (Comment) Organism
gene GalUR, recombinant overexpression in Solanum lycopersicum cv. Ailsa Craig leaves and fruits via Agrobacterium tumefaciens strain C58C1 transfection resulting in 2fold and 1.6fold increase in ascorbate level in tomato fruit and leaf, respectively, which correlates positively with FaGalUR transcriptional abundance and enzyme GalUR activity compared to wild-type plants, real-time PCR expression analysis Fragaria x ananassa

Protein Variants

Protein Variants Comment Organism
additional information gene FaGalUR-overexpressing Solanum lycopersicum plants show enhanced ascorbic acid levels, tolerance to abiotic stresses induced by oxidization (methyl viologen), salt (NaCl), and cold compared to the wild-type plants, overview. Ascorbate accumulation in tomato can be enhanced by regulating Fragaria x ananassa GalUR gene Fragaria x ananassa

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galacturonate + NADPH + H+ Fragaria x ananassa
-
L-galactonate + NADP+
-
r

Organism

Organism UniProt Comment Textmining
Fragaria x ananassa O49133 cv. Fenglaixiang, Fragaria chiloensis x Fragaria virginiana
-

Source Tissue

Source Tissue Comment Organism Textmining
fruit
-
Fragaria x ananassa
-
leaf
-
Fragaria x ananassa
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galacturonate + NADPH + H+
-
Fragaria x ananassa L-galactonate + NADP+
-
r

Synonyms

Synonyms Comment Organism
FaGalUR
-
Fragaria x ananassa
GalUR
-
Fragaria x ananassa

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
assay at Fragaria x ananassa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.2
-
assay at Fragaria x ananassa

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Fragaria x ananassa
NADPH
-
Fragaria x ananassa

General Information

General Information Comment Organism
physiological function the D-galacturonic acid reductase catalyzes the conversion of D-galacturonic acid to L-galactonic acid in plant. The D-galacturonic acid, known as an abundant component of the cell wall, is a degradation product of pectin in senescencing plant cells. The expression levels of FaGalUR are correlated with increased ascorbate content in ripe strawberry fruit. Fragaria x ananassa gene FaGalUR expression improves salt and cold tolerance in Solanum lycopersicum fruits, and improves tolerance to oxidative stress in tomato Fragaria x ananassa