Application | Comment | Organism |
---|---|---|
nutrition | accumulation of high levels of ascorbate using GalUR gene overepression in tomato fruits. Tomato fruits are considered a major dietary source of vitamin C in many countries, because it is consumed regularly and in large quantities. Tomato also serves as a fruit model for other crops species with fleshy berry. Accordingly, it is very important to monitor and to increase the vitamin C content in tomato fruit, for meeting the consumer demand and health requirements for high nutrition, e.g. by overexpressing D-galacturonate reductase | Fragaria x ananassa |
Cloned (Comment) | Organism |
---|---|
gene GalUR, recombinant overexpression in Solanum lycopersicum cv. Ailsa Craig leaves and fruits via Agrobacterium tumefaciens strain C58C1 transfection resulting in 2fold and 1.6fold increase in ascorbate level in tomato fruit and leaf, respectively, which correlates positively with FaGalUR transcriptional abundance and enzyme GalUR activity compared to wild-type plants, real-time PCR expression analysis | Fragaria x ananassa |
Protein Variants | Comment | Organism |
---|---|---|
additional information | gene FaGalUR-overexpressing Solanum lycopersicum plants show enhanced ascorbic acid levels, tolerance to abiotic stresses induced by oxidization (methyl viologen), salt (NaCl), and cold compared to the wild-type plants, overview. Ascorbate accumulation in tomato can be enhanced by regulating Fragaria x ananassa GalUR gene | Fragaria x ananassa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonate + NADPH + H+ | Fragaria x ananassa | - |
L-galactonate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Fragaria x ananassa | O49133 | cv. Fenglaixiang, Fragaria chiloensis x Fragaria virginiana | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
fruit | - |
Fragaria x ananassa | - |
leaf | - |
Fragaria x ananassa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonate + NADPH + H+ | - |
Fragaria x ananassa | L-galactonate + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
FaGalUR | - |
Fragaria x ananassa |
GalUR | - |
Fragaria x ananassa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Fragaria x ananassa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
assay at | Fragaria x ananassa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Fragaria x ananassa | |
NADPH | - |
Fragaria x ananassa |
General Information | Comment | Organism |
---|---|---|
physiological function | the D-galacturonic acid reductase catalyzes the conversion of D-galacturonic acid to L-galactonic acid in plant. The D-galacturonic acid, known as an abundant component of the cell wall, is a degradation product of pectin in senescencing plant cells. The expression levels of FaGalUR are correlated with increased ascorbate content in ripe strawberry fruit. Fragaria x ananassa gene FaGalUR expression improves salt and cold tolerance in Solanum lycopersicum fruits, and improves tolerance to oxidative stress in tomato | Fragaria x ananassa |