Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.1.1.365 extracted from

  • Sloothaak, J.; Schilders, M.; Schaap, P.J.; de Graaff, L.H.
    Overexpression of the Aspergillus niger GatA transporter leads to preferential use of D-galacturonic acid over D-xylose (2014), AMB Express, 4, 66 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.1.1.365

Cloned(Commentary)

Cloned (Comment) Organism
gene gaaA, expression analysis in wild-type strain and in a strain overexpressing the D-galacturonic acid-specific GatA transporter Aspergillus niger

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-galacturonate + NADPH + H+ Aspergillus niger
-
 L-galactonate + NADP+
-
 r
D-galacturonate + NADPH + H+ Aspergillus niger CBS 120.49
-
 L-galactonate + NADP+
-
 r

Organism

Organism UniProt Comment Textmining
Aspergillus niger A8DRH9
-
-
Aspergillus niger CBS 120.49 A8DRH9
-
-

Source Tissue

Source Tissue Comment Organism Textmining
mycelium
-
 Aspergillus niger
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-galacturonate + NADPH + H+
-
 Aspergillus niger L-galactonate + NADP+
-
 r
D-galacturonate + NADPH + H+
-
 Aspergillus niger CBS 120.49 L-galactonate + NADP+
-
 r

Synonyms

Synonyms Comment Organism
gaaA
-
 Aspergillus niger

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Aspergillus niger

Cofactor

Cofactor Comment Organism Structure
NADP+
-
 Aspergillus niger
NADPH
-
 Aspergillus niger

General Information

General Information Comment Organism
metabolism after import via the D-galacturonic acid transporter encoded by An14g04280, D-galacturonic acid is catabolized by three key enzymes: D-galacturonic acid reductase, L-galactonate dehydratase, and 2-keto-3-deoxy-galactonate aldolase, in Aspegillus niger. The first step in the D-galacturonic acid metabolism is the enzymatic conversion of D-galacturonic acid to L-galactonic acid by D-galacturonic acid reductase. Overexpression of the Aspergillus niger GatA transporter leads to preferential uptake of D-galacturonic acid over D-xylose of mutant strain JS013 and enanced use of D-galacturonic acid compared to D-xylose. Increased activity of the D-galacturonic acid metabolic pathway is observed for strain JS013 transformant strain in comparison to the control Aspergillus niger