Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli DH5alpha cells | Aspergillus niger |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.036 | - |
NADPH | with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
0.175 | - |
D-galacturonate | with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
0.326 | - |
NADH | with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
7.11 | - |
D-galacturonate | with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44000 | - |
x * 44000, His6-tagged enzyme, SDS-PAGE | Aspergillus niger |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonate + NADPH + H+ | Aspergillus niger | - |
L-galactonate + NADP+ | - |
? | |
D-galacturonate + NADPH + H+ | Aspergillus niger CBS 120.49 | - |
L-galactonate + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Aspergillus niger | - |
- |
- |
Aspergillus niger CBS 120.49 | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
mycelium | - |
Aspergillus niger | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.12 | - |
with NAD+ and L-galactonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
0.17 | - |
with NADH and D-glucuronate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
0.21 | - |
with NADP+ and L-galactonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
3.9 | - |
with NADPH and D-glucuronate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
6.9 | - |
with NADH and D-galacturonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
8.7 | - |
with NADPH and D-galacturonate as substrates, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonate + NADH + H+ | - |
Aspergillus niger | L-galactonate + NAD+ | - |
r | |
D-galacturonate + NADH + H+ | - |
Aspergillus niger CBS 120.49 | L-galactonate + NAD+ | - |
r | |
D-galacturonate + NADPH + H+ | - |
Aspergillus niger | L-galactonate + NADP+ | - |
? | |
D-galacturonate + NADPH + H+ | - |
Aspergillus niger | L-galactonate + NADP+ | - |
r | |
D-galacturonate + NADPH + H+ | - |
Aspergillus niger CBS 120.49 | L-galactonate + NADP+ | - |
? | |
D-galacturonate + NADPH + H+ | - |
Aspergillus niger CBS 120.49 | L-galactonate + NADP+ | - |
r | |
D-glucuronate + NADH + H+ | - |
Aspergillus niger | ? | - |
? | |
D-glucuronate + NADH + H+ | - |
Aspergillus niger CBS 120.49 | ? | - |
? | |
D-glucuronate + NADPH + H+ | - |
Aspergillus niger | ? | - |
? | |
D-glucuronate + NADPH + H+ | - |
Aspergillus niger CBS 120.49 | ? | - |
? | |
L-galactonate + NAD+ | - |
Aspergillus niger | D-galacturonate + NADH + H+ | - |
r | |
L-galactonate + NADP+ | - |
Aspergillus niger | D-galacturonate + NADPH + H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 44000, His6-tagged enzyme, SDS-PAGE | Aspergillus niger |
Synonyms | Comment | Organism |
---|---|---|
gaaA | - |
Aspergillus niger |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
L-galactonate | with NAD+ as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
0.13 | - |
D-glucuronate | with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
0.16 | - |
L-galactonate | with NADP+ as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
3.11 | - |
D-glucuronate | with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
4.98 | - |
NADH | with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
5.45 | - |
D-galacturonate | with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
6.88 | - |
D-galacturonate | with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
7.11 | - |
NADPH | with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Aspergillus niger | |
NADPH | preferred cofactor | Aspergillus niger |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.767 | - |
D-galacturonate | with NADH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
15.72 | - |
NADH | with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
39.29 | - |
D-galacturonate | with NADPH as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger | |
197.2 | - |
NADPH | with D-galacturonate as cosubstrate, in 10 mM sodium phosphate, at pH 7.2 and 22°C | Aspergillus niger |