Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 cells | Botrytis cinerea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.12 | - |
D-glucuronate | isoform GAR1, 10 mM sodium phosphate, pH 7.0, at 22°C | Botrytis cinerea | |
1 | - |
D-glucuronate | isoform GAR2, 10 mM sodium phosphate, pH 7.0, at 22°C | Botrytis cinerea | |
1.4 | - |
D-galacturonate | isoform GAR2, 10 mM sodium phosphate, pH 7.0, at 22°C | Botrytis cinerea | |
2.5 | - |
D-galacturonate | isoform GAR1, 10 mM sodium phosphate, pH 7.0, at 22°C | Botrytis cinerea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
36900 | - |
x * 36900, isoform GAR1, calculated from amino acid sequence | Botrytis cinerea |
49200 | - |
x * 49200, isoform GAR2, calculated from amino acid sequence | Botrytis cinerea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonate + NADPH + H+ | Botrytis cinerea | - |
L-galactonate + NADP+ | - |
r | |
D-galacturonate + NADPH + H+ | Botrytis cinerea B05.10 | - |
L-galactonate + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Botrytis cinerea | - |
- |
- |
Botrytis cinerea B05.10 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-galacturonate + NADPH + H+ | - |
Botrytis cinerea | L-galactonate + NADP+ | - |
r | |
D-galacturonate + NADPH + H+ | - |
Botrytis cinerea B05.10 | L-galactonate + NADP+ | - |
r | |
D-glucuronate + NADPH + H+ | - |
Botrytis cinerea | ? | - |
r | |
D-glucuronate + NADPH + H+ | - |
Botrytis cinerea B05.10 | ? | - |
r |
Subunits | Comment | Organism |
---|---|---|
? | x * 36900, isoform GAR1, calculated from amino acid sequence | Botrytis cinerea |
? | x * 49200, isoform GAR2, calculated from amino acid sequence | Botrytis cinerea |
Synonyms | Comment | Organism |
---|---|---|
gar1 | isoform | Botrytis cinerea |
GAR2 | isoform | Botrytis cinerea |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Botrytis cinerea |
Organism | Comment | Expression |
---|---|---|
Botrytis cinerea | isoform GAR1 is induced about 10fold, while isoform GAR2 is strongly induced in cultures with D-galacturonic acid, pectate, and pectin as carbon source at 3 h after transfer, compared to continuous growth in glucose-containing culture | up |