Cloned (Comment) | Organism |
---|---|
gene phbB, functional expression in Escherichia coli strains BW25113 and DH5alpha, co-expression with genes phbA and tesB, encoding beta-ketothiolase and thioesterase II, respectively, to reconstruct the R-3-hydroxybutyric acid production pathway, optimization of culture conditions, overview | Cupriavidus necator |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutyryl-CoA + NADPH + H+ | Cupriavidus necator | - |
3-oxobutyryl-CoA + NADP+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Cupriavidus necator | - |
gene phbB | - |
no activity in Escherichia coli | - |
strain DH5alpha | - |
no activity in Escherichia coli DH5-alpha | - |
strain DH5alpha | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.53 | 0.62 | recombinant enzyme in transformed Escherichia coli strain DH5alpha | Cupriavidus necator |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-3-hydroxybutyryl-CoA + NADPH + H+ | - |
Cupriavidus necator | 3-oxobutyryl-CoA + NADP+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
acetoacetyl CoA reductase | - |
Cupriavidus necator |
PhbB | - |
Cupriavidus necator |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Cupriavidus necator |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Cupriavidus necator |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADP+ | - |
Cupriavidus necator | |
NADPH | - |
Cupriavidus necator |