Inhibitors | Comment | Organism | Structure |
---|---|---|---|
acetoacetyl-CoA | NADH-linked, less sensitive; NADPH-linked, concentration above 0.025 mM, nearly independent of NADPH concentrations 0.03-0.5 mM | Methylorubrum rhodesianum | |
acetoacetyl-CoA | - |
Zoogloea ramigera | |
additional information | NADH-linked: not inhibited by NADPH at concentrations of 1 mM, NADPH-linked: not inhibited by NAD+ at concentrations of 1 mM | Methylorubrum rhodesianum | |
NAD+ | NADH-linked | Methylorubrum rhodesianum | |
NADP+ | - |
Methylorubrum rhodesianum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.015 | - |
acetoacetyl-CoA | cofactor NADPH | Methylorubrum rhodesianum | |
0.018 | - |
NADPH | - |
Methylorubrum rhodesianum | |
0.022 | - |
L-beta-hydroxybutyryl-CoA | - |
Methylorubrum rhodesianum | |
0.03 | - |
D-beta-hydroxybutyryl-CoA | - |
Methylorubrum rhodesianum | |
0.03 | - |
acetoacetyl-CoA | cofactor NADH | Methylorubrum rhodesianum | |
0.06 | - |
NADP+ | - |
Methylorubrum rhodesianum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
42000 | - |
NADH-linked enzyme: x * 55000, NADPH-linked enzyme: x * 42000, SDS-PAGE | Methylorubrum rhodesianum |
250000 | - |
NADPH-linked: gel filtration | Methylorubrum rhodesianum |
340000 | - |
NADH-linked: gel filtration | Methylorubrum rhodesianum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADH + H+ | Zoogloea ramigera | - |
L-3-hydroxybutyryl-CoA + NAD+ | - |
? | |
acetoacetyl-CoA + NADH + H+ | Rhodospirillum rubrum | - |
L-3-hydroxybutyryl-CoA + NAD+ | - |
? | |
acetoacetyl-CoA + NADPH + H+ | Zoogloea ramigera | PHB synthesis | D-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
acetoacetyl-CoA + NADPH + H+ | Rhodococcus ruber | PHB synthesis | D-3-hydroxybutyryl-CoA + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylorubrum rhodesianum | - |
MB 126 | - |
Methylorubrum rhodesianum MB 126 | - |
MB 126 | - |
Rhodococcus ruber | - |
- |
- |
Rhodospirillum rubrum | - |
- |
- |
Zoogloea ramigera | - |
- |
- |
Purification (Comment) | Organism |
---|---|
NADH-linked: DEAE-Sepharose CL-6B, Blue Sepharose CL-6B, botyl Sepharose 4B, Mono Q 5/5 | Methylorubrum rhodesianum |
NADPH-linked: DEAE-Sepharose CL-6B, Red 120-agarose, phenyle Sepharose | Methylorubrum rhodesianum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
400 | - |
NADPH-linked | Methylorubrum rhodesianum |
771 | - |
NADH-linked, 345 with NADPH | Methylorubrum rhodesianum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
acetoacetyl-CoA + NADH + H+ | reverse only with L-form | Methylorubrum rhodesianum | L-beta-hydroxybutyryl-CoA + NAD+ | - |
r | |
acetoacetyl-CoA + NADH + H+ | reverse only with L-form | Methylorubrum rhodesianum MB 126 | L-beta-hydroxybutyryl-CoA + NAD+ | - |
r | |
acetoacetyl-CoA + NADH + H+ | - |
Zoogloea ramigera | L-3-hydroxybutyryl-CoA + NAD+ | - |
? | |
acetoacetyl-CoA + NADH + H+ | - |
Rhodospirillum rubrum | L-3-hydroxybutyryl-CoA + NAD+ | - |
? | |
acetoacetyl-CoA + NADPH + H+ | reverse only with D-form | Methylorubrum rhodesianum | D-beta-hydroxybutyryl-CoA + NADP+ | - |
r | |
acetoacetyl-CoA + NADPH + H+ | reverse only with D-form | Methylorubrum rhodesianum MB 126 | D-beta-hydroxybutyryl-CoA + NADP+ | - |
r | |
acetoacetyl-CoA + NADPH + H+ | PHB synthesis | Zoogloea ramigera | D-3-hydroxybutyryl-CoA + NADP+ | - |
? | |
acetoacetyl-CoA + NADPH + H+ | PHB synthesis | Rhodococcus ruber | D-3-hydroxybutyryl-CoA + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | NADH-linked enzyme: x * 55000, NADPH-linked enzyme: x * 42000, SDS-PAGE | Methylorubrum rhodesianum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
NADPH-linked, reverse reaction pH 10.0 | Methylorubrum rhodesianum |
7 | - |
NADH-linked, reverse reaction pH 9.5 | Methylorubrum rhodesianum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADH | - |
Zoogloea ramigera | |
NADH | - |
Rhodospirillum rubrum | |
NADH | - |
Methylorubrum rhodesianum | |
NADPH | - |
Zoogloea ramigera | |
NADPH | - |
Rhodococcus ruber | |
NADPH | - |
Methylorubrum rhodesianum |