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Literature summary for 1.1.1.358 extracted from

  • Cheng, P.; Tang, M.; Chen, Z.; Liu, W.; Jiang, X.; Pei, X.; Su, W.
    Dual-enzyme and NADPH co-embedded organic-inorganic hybrid nanoflowers prepared using biomimetic mineralization for the asymmetric synthesis of ( R)-(-)-pantolactone (2020), React. Chem. Eng., 5, 1973-1980 .
No PubMed abstract available

Cloned(Commentary)

Cloned (Comment) Organism
gene CPR-C2, functional overexpression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Candida dubliniensis

Protein Variants

Protein Variants Comment Organism
additional information an effective biocatalytic cascade was developed by encapsulating a conjugated polyketone reductase (CPR), glucose dehydrogenase (GDH) and the coenzyme NADP+ in organic-inorganic hybrid nanoflowers (hNFs) for the asymmetric reduction of oxopantolactone (KPL) to synthesize (R)-(-)-pantolactone ((R)-PL). When CduCPR and TgGDH are confined in the sodium alginate (SA)-coated hNFs [CduCPR/TgGDH-Ca3(PO4)2], both of them have excellent reusability and high stability. The SA-coated hNF reactor successfully catalyzes the asymmetric synthesis of (R)-PL, which exhibits satisfactory stereoselectivity and promotes reusability in repeated batches. The SA coating functioned to competently immobilize the coenzyme NADP+ on the CduCPR/TgGDH-Ca3(PO4)2 hNFs, which maintains the maximum bioactivity to synthesize (R)-PL without exogenous coenzyme addition. Method development, optimization, and evaluation, overview Candida dubliniensis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.484
-
2-dehydropantolactone pH 7.0, 30°C, recombinant free enzyme Candida dubliniensis
0.886
-
2-dehydropantolactone pH 7.0, 30°C, recombinant immobilized enzyme Candida dubliniensis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydropantolactone + NADPH + H+ Candida dubliniensis
-
(R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+ Candida dubliniensis ATCC MYA-646
-
(R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+ Candida dubliniensis CD36
-
(R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+ Candida dubliniensis NCPF 3949
-
(R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+ Candida dubliniensis NRRL Y-17841
-
(R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+ Candida dubliniensis CBS 7987
-
(R)-pantolactone + NADP+
-
?

Organism

Organism UniProt Comment Textmining
Candida dubliniensis B9WJQ5
-
-
Candida dubliniensis ATCC MYA-646 B9WJQ5
-
-
Candida dubliniensis CBS 7987 B9WJQ5
-
-
Candida dubliniensis CD36 B9WJQ5
-
-
Candida dubliniensis NCPF 3949 B9WJQ5
-
-
Candida dubliniensis NRRL Y-17841 B9WJQ5
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis Candida dubliniensis

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
22
-
purified immobilized enzyme CduCPR, pH 7.0, 30°C Candida dubliniensis
49.1
-
purified free enzyme CduCPR, pH 7.0, 30°C Candida dubliniensis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydropantolactone + NADPH + H+
-
Candida dubliniensis (R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+
-
Candida dubliniensis ATCC MYA-646 (R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+
-
Candida dubliniensis CD36 (R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+
-
Candida dubliniensis NCPF 3949 (R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+
-
Candida dubliniensis NRRL Y-17841 (R)-pantolactone + NADP+
-
?
2-dehydropantolactone + NADPH + H+
-
Candida dubliniensis CBS 7987 (R)-pantolactone + NADP+
-
?
additional information product identification and quantification by gas chromatography. Optimization of the reaction conditions for the synthesis of (R)-(-)-pantolactone Candida dubliniensis ?
-
-
additional information product identification and quantification by gas chromatography. Optimization of the reaction conditions for the synthesis of (R)-(-)-pantolactone Candida dubliniensis ATCC MYA-646 ?
-
-
additional information product identification and quantification by gas chromatography. Optimization of the reaction conditions for the synthesis of (R)-(-)-pantolactone Candida dubliniensis CD36 ?
-
-
additional information product identification and quantification by gas chromatography. Optimization of the reaction conditions for the synthesis of (R)-(-)-pantolactone Candida dubliniensis NCPF 3949 ?
-
-
additional information product identification and quantification by gas chromatography. Optimization of the reaction conditions for the synthesis of (R)-(-)-pantolactone Candida dubliniensis NRRL Y-17841 ?
-
-
additional information product identification and quantification by gas chromatography. Optimization of the reaction conditions for the synthesis of (R)-(-)-pantolactone Candida dubliniensis CBS 7987 ?
-
-

Synonyms

Synonyms Comment Organism
CduCPR
-
Candida dubliniensis
conjugated polyketone reductase
-
Candida dubliniensis
CPR
-
Candida dubliniensis
CPR-C2
-
Candida dubliniensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40 45 recombinant enzyme Candida dubliniensis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
13.6
-
2-dehydropantolactone pH 7.0, 30°C, recombinant immobilized enzyme Candida dubliniensis
59
-
2-dehydropantolactone pH 7.0, 30°C, recombinant free enzyme Candida dubliniensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
recombinant enzyme Candida dubliniensis

Cofactor

Cofactor Comment Organism Structure
NADPH
-
Candida dubliniensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
15.35
-
2-dehydropantolactone pH 7.0, 30°C, recombinant immobilized enzyme Candida dubliniensis
121.9
-
2-dehydropantolactone pH 7.0, 30°C, recombinant free enzyme Candida dubliniensis