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Literature summary for 1.1.1.358 extracted from

  • Qin, H.M.; Yamamura, A.; Miyakawa, T.; Kataoka, M.; Maruoka, S.; Ohtsuka, J.; Nagata, K.; Shimizu, S.; Tanokura, M.
    Crystal structure of conjugated polyketone reductase (CPR-C1) from Candida parapsilosis IFO 0708 complexed with NADPH (2013), Proteins, 81, 2059-2063.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta(DE3) cells Candida parapsilosis

Crystallization (Commentary)

Crystallization (Comment) Organism
sitting drop vapor diffusion method, using 0.1 M Tris-HCl (pH 8.5), 25% (w/v) PEG3350, and 0.2 M NaCl Candida parapsilosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydropantolactone + NADPH + H+ Candida parapsilosis
-
 (R)-pantolactone + NADP+
-
 ?
2-dehydropantolactone + NADPH + H+ Candida parapsilosis IFO 0708
-
 (R)-pantolactone + NADP+
-
 ?

Organism

Organism UniProt Comment Textmining
Candida parapsilosis Q76L36
-
-
Candida parapsilosis IFO 0708 Q76L36
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration Candida parapsilosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydropantolactone + NADPH + H+
-
 Candida parapsilosis (R)-pantolactone + NADP+
-
 ?
2-dehydropantolactone + NADPH + H+
-
 Candida parapsilosis IFO 0708 (R)-pantolactone + NADP+
-
 ?

Synonyms

Synonyms Comment Organism
conjugated polyketone reductase
-
 Candida parapsilosis
CPR-C1
-
 Candida parapsilosis

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Candida parapsilosis