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Literature summary for 1.1.1.358 extracted from

  • Qin, H.M.; Yamamura, A.; Miyakawa, T.; Kataoka, M.; Nagai, T.; Kitamura, N.; Urano, N.; Maruoka, S.; Ohtsuka, J.; Nagata, K.; Shimizu, S.; Tanokura, M.
    Structure of conjugated polyketone reductase from Candida parapsilosis IFO 0708 reveals conformational changes for substrate recognition upon NADPH binding (2013), Appl. Microbiol. Biotechnol., 98, 243-249.
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta (DE3) cells Candida parapsilosis

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme and in complex with NADPH, sitting drop vapor diffusion method, using 0.1 M Tris–HCl (pH 8.1) and 23% (w/v) polyethylene glycol 3350, at 20°C Candida parapsilosis

Protein Variants

Protein Variants Comment Organism
D58A 4.82% activity compared to the wild type enzyme Candida parapsilosis
F299A 19.1% activity compared to the wild type enzyme Candida parapsilosis
F300A 17.8% activity compared to the wild type enzyme Candida parapsilosis
H125A 1.5% activity compared to the wild type enzyme Candida parapsilosis
K264A 65.7% activity compared to the wild type enzyme Candida parapsilosis
K28A 71.1% activity compared to the wild type enzyme Candida parapsilosis
K30A 55.1% activity compared to the wild type enzyme Candida parapsilosis
K88A inactive Candida parapsilosis
R267A 8.43% activity compared to the wild type enzyme Candida parapsilosis
T27A 5.75% activity compared to the wild type enzyme Candida parapsilosis
Y63A 0.17% activity compared to the wild type enzyme Candida parapsilosis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2-dehydropantolactone + NADPH + H+ Candida parapsilosis
-
 (R)-pantolactone + NADP+
-
 ir
2-dehydropantolactone + NADPH + H+ Candida parapsilosis IFO 0708
-
 (R)-pantolactone + NADP+
-
 ir

Organism

Organism UniProt Comment Textmining
Candida parapsilosis Q76L36
-
-
Candida parapsilosis IFO 0708 Q76L36
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni Sepharose column chromatography, Resource Q column chromatography, and Superdex 75 gel filtration Candida parapsilosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2-dehydropantolactone + NADPH + H+
-
 Candida parapsilosis (R)-pantolactone + NADP+
-
 ir
2-dehydropantolactone + NADPH + H+
-
 Candida parapsilosis IFO 0708 (R)-pantolactone + NADP+
-
 ir

Synonyms

Synonyms Comment Organism
conjugated polyketone reductase C2
-
 Candida parapsilosis
CPR-C2
-
 Candida parapsilosis
NADPH-dependent ketopantoyl lactone reductase
-
 Candida parapsilosis

Cofactor

Cofactor Comment Organism Structure
NADPH dependent on Candida parapsilosis