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Literature summary for 1.1.1.35 extracted from

  • Segawa, M.; Wen, C.; Orita, I.; Nakamura, S.; Fukui, T.
    Two NADH-dependent (S)-3-hydroxyacyl-CoA dehydrogenases from polyhydroxyalkanoate-producing Ralstonia eutropha (2019), J. Biosci. Bioeng., 127, 294-300 .
    View publication on PubMed
Search for more literature for 1.1.1.35

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Cupriavidus necator

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0065
-
 3-oxooctanoyl-CoA enzyme Had, at pH 8.0 and 30°C Cupriavidus necator
0.0083
-
 3-oxohexanoyl-CoA enzyme PaaH1, at pH 8.0 and 30°C Cupriavidus necator
0.0094
-
 3-acetoacyl-CoA enzyme PaaH1, at pH 8.0 and 30°C Cupriavidus necator
0.0125
-
 3-oxooctanoyl-CoA enzyme PaaH1, at pH 8.0 and 30°C Cupriavidus necator
0.016
-
 3-oxohexanoyl-CoA enzyme Had, at pH 8.0 and 30°C Cupriavidus necator
0.0175
-
 3-acetoacyl-CoA enzyme Had, at pH 8.0 and 30°C Cupriavidus necator

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-oxoacyl-CoA + NADH + H+ Cupriavidus necator
-
 (S)-3-hydroxyacyl-CoA + NAD+
-
 ?

Organism

Organism UniProt Comment Textmining
Cupriavidus necator
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Q Sepharose column chromatography, HiTrap phenyl column chromatography and Superdex 200 gel filtration Cupriavidus necator

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-acetoacyl-CoA + NADH + H+ highest activity Cupriavidus necator (S)-3-hydroxybutyryl-CoA + NAD+
-
 ?
3-oxoacyl-CoA + NADH + H+
-
 Cupriavidus necator (S)-3-hydroxyacyl-CoA + NAD+
-
 ?
3-oxohexanoyl-CoA + NADH + H+
-
 Cupriavidus necator (S)-3-hydroxyhexanoyl-CoA + NAD+
-
 ?
3-oxooctanoyl-CoA + NADH + H+
-
 Cupriavidus necator (S)-3-hydroxyoctanoyl-CoA + NAD+
-
 ?
additional information the activity of enzymes PaaH1 and Had with NADPH is as low as 2.2% and 1.2%, respectively, of the respective activity with NADH Cupriavidus necator ?
-
-

Subunits

Subunits Comment Organism
? x * 30000, SDS-PAGE Cupriavidus necator

Synonyms

Synonyms Comment Organism
3HA-CoA dehydrogenase
-
 Cupriavidus necator
HAD
-
 Cupriavidus necator
NADH-3HB-CoA dehydrogenase
-
 Cupriavidus necator
NADH-dependent (S)-3-hydroxyacyl-CoA dehydrogenase
-
 Cupriavidus necator
NADH-dependent 3-hydroxyacyl-CoA dehydrogenase
-
 Cupriavidus necator
PaaH1
-
 Cupriavidus necator

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
11
-
 3-oxooctanoyl-CoA enzyme Had, at pH 8.0 and 30°C Cupriavidus necator
40
-
 3-oxohexanoyl-CoA enzyme Had, at pH 8.0 and 30°C Cupriavidus necator
43
-
 3-oxohexanoyl-CoA enzyme PaaH1, at pH 8.0 and 30°C Cupriavidus necator
53
-
 3-oxooctanoyl-CoA enzyme PaaH1, at pH 8.0 and 30°C Cupriavidus necator
84
-
 3-acetoacyl-CoA enzyme PaaH1, at pH 8.0 and 30°C Cupriavidus necator
104
-
 3-acetoacyl-CoA enzyme Had, at pH 8.0 and 30°C Cupriavidus necator

Cofactor

Cofactor Comment Organism Structure
NADH
-
 Cupriavidus necator

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1700
-
 3-oxooctanoyl-CoA enzyme Had, at pH 8.0 and 30°C Cupriavidus necator
2500
-
 3-oxohexanoyl-CoA enzyme Had, at pH 8.0 and 30°C Cupriavidus necator
4200
-
 3-oxooctanoyl-CoA enzyme PaaH1, at pH 8.0 and 30°C Cupriavidus necator
5200
-
 3-oxohexanoyl-CoA enzyme PaaH1, at pH 8.0 and 30°C Cupriavidus necator
6000
-
 3-acetoacyl-CoA enzyme Had, at pH 8.0 and 30°C Cupriavidus necator
8900
-
 3-acetoacyl-CoA enzyme PaaH1, at pH 8.0 and 30°C Cupriavidus necator