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Literature summary for 1.1.1.35 extracted from

  • Takenoya, M.; Taguchi, S.; Yajima, S.
    Crystal structure and kinetic analyses of a hexameric form of (S)-3-hydroxybutyryl-CoA dehydrogenase from Clostridium acetobutylicum (2018), Acta Crystallogr. Sect. F, 74, 733-740 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli Rosetta 2(DE3) cells Clostridium acetobutylicum

Crystallization (Commentary)

Crystallization (Comment) Organism
apoenzyme, hanging drop vapor diffusion method, using 1.25% (w/v) PEG 400, 2.2 M ammonium sulfate, 0.1 M HEPES-NaOH pH 7.5. Enzyme in complex with NAD+, hanging drop vapor diffusion method, using 17.5% (w/v) PEG 3350, 200 mM sodium thiocyanate Clostridium acetobutylicum

Protein Variants

Protein Variants Comment Organism
H138A the mutant shows a slightly lower Km value and a significantly lower kcat value than the wild type enzyme Clostridium acetobutylicum
N188A the mutation abolishes the activity of the enzyme Clostridium acetobutylicum
S117A the mutation abolishes the activity of the enzyme Clostridium acetobutylicum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0096
-
3-acetoacetyl-CoA mutant enzyme H138A, at pH 7.5 and 37°C Clostridium acetobutylicum
0.0168
-
NADH mutant enzyme H138A, at pH 7.5 and 37°C Clostridium acetobutylicum
0.0227
-
3-acetoacetyl-CoA wild type enzyme, at pH 7.5 and 37°C Clostridium acetobutylicum
0.0314
-
NADH wild type enzyme, at pH 7.5 and 37°C Clostridium acetobutylicum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3-acetoacetyl-CoA + NADH + H+ Clostridium acetobutylicum
-
(S)-3-hydroxybutanoyl-CoA + NAD+
-
?

Organism

Organism UniProt Comment Textmining
Clostridium acetobutylicum
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni column chromatography and Mono Q column chromatography Clostridium acetobutylicum

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3-acetoacetyl-CoA + NADH + H+
-
Clostridium acetobutylicum (S)-3-hydroxybutanoyl-CoA + NAD+
-
?

Subunits

Subunits Comment Organism
hexamer x-ray crystallography Clostridium acetobutylicum

Synonyms

Synonyms Comment Organism
(S)-3-hydroxybutyryl-CoA dehydrogenase
-
Clostridium acetobutylicum
HBD
-
Clostridium acetobutylicum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
93.8
-
NADH mutant enzyme H138A, at pH 7.5 and 37°C Clostridium acetobutylicum
104.3
-
3-acetoacetyl-CoA mutant enzyme H138A, at pH 7.5 and 37°C Clostridium acetobutylicum
8500
-
NADH wild type enzyme, at pH 7.5 and 37°C Clostridium acetobutylicum
13000
-
3-acetoacetyl-CoA wild type enzyme, at pH 7.5 and 37°C Clostridium acetobutylicum

Cofactor

Cofactor Comment Organism Structure
NADH
-
Clostridium acetobutylicum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.0056
-
NADH mutant enzyme H138A, at pH 7.5 and 37°C Clostridium acetobutylicum
0.011
-
3-acetoacetyl-CoA mutant enzyme H138A, at pH 7.5 and 37°C Clostridium acetobutylicum
0.27
-
NADH wild type enzyme, at pH 7.5 and 37°C Clostridium acetobutylicum
0.63
-
3-acetoacetyl-CoA wild type enzyme, at pH 7.5 and 37°C Clostridium acetobutylicum