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Literature summary for 1.1.1.34 extracted from

  • Hedl, M.; Sutherlin, A.; Wilding, E.I.; Mazzulla, M.; McDevitt, D.; Lane, P.; Burgner, J.W.; Lehnbeuter, K.R.; Stauffacher, C.V.; Gwynn, M.N.; Rodwell, V.W.
    Enterococcus faecalis acetoacetyl-coenzyme A thiolase/3-hydroxy-3-methylglutaryl-coenzyme A reductase, a dual-function protein of isopentenyl diphosphate biosynthesis (2002), J. Bacteriol., 184, 2116-2122.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli strain DH5alpha as His-tagged enzyme Enterococcus faecalis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.02
-
(S)-3-hydroxy-3-methylglutaryl-CoA pH 6.5, 37°C Enterococcus faecalis
0.03
-
NADPH pH 6.5, 37°C, (S)-3-hydroxy-3-methylglutaryl-CoA reduction Enterococcus faecalis
0.22
-
CoA pH 6.5, 37°C, mevaldehyde oxidation Enterococcus faecalis
0.23
-
CoA pH 6.5, 37°C, mevalonate oxidation Enterococcus faecalis
0.25
-
NADPH pH 6.5, 37°C, mevaldehyde reduction Enterococcus faecalis
0.25
-
NADP+ pH 6.5, 37°C, mevalonate oxidation Enterococcus faecalis
0.66
-
mevaldehyde pH 6.5, 37°C, oxidation Enterococcus faecalis
0.67
-
NADP+ pH 6.5, 37°C, mevaldehyde oxidation Enterococcus faecalis
1
-
(R)-mevalonate pH 6.5, 37°C, oxidation Enterococcus faecalis
3.8
-
mevaldehyde pH 6.5, 37°C, reduction Enterococcus faecalis

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ Enterococcus faecalis involved in isopentenyl diphosphate biosynthesis, mevalonate pathway overview (R)-mevalonate + CoA + 2 NADP+
-
r
acetyl-CoA Enterococcus faecalis
-
acetoacetyl-CoA + CoA
-
r

Organism

Organism UniProt Comment Textmining
Enterococcus faecalis
-
dual-function enzyme with acetoacetyl-coenzyme A thiolase and 3-hydroxy-3-methylglutaryl-coenzyme A reductase activities
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged enzyme from Escherichia coli by nickel-affinity chromatography Enterococcus faecalis

Reaction

Reaction Comment Organism Reaction ID
(R)-mevalonate + CoA + 2 NADP+ = (S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ via mevaldehyde as an intermediate product Enterococcus faecalis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ involved in isopentenyl diphosphate biosynthesis, mevalonate pathway overview Enterococcus faecalis (R)-mevalonate + CoA + 2 NADP+
-
r
(S)-3-hydroxy-3-methylglutaryl-CoA + 2 NADPH + 2 H+ overall reaction Enterococcus faecalis (R)-mevalonate + CoA + 2 NADP+
-
r
(S)-3-hydroxy-3-methylglutaryl-CoA + NADPH + H+ first step reaction Enterococcus faecalis mevaldehyde + CoA + NADP+
-
r
acetyl-CoA
-
Enterococcus faecalis acetoacetyl-CoA + CoA
-
r
mevaldehyde + NADPH + H+ second step reaction Enterococcus faecalis (R)-mevalonate + NADP+
-
r

Synonyms

Synonyms Comment Organism
acetoacetyl-coenzyme A thiolase/3-hydroxy-3-methylglutaryl-coenzyme A reductase
-
Enterococcus faecalis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
about, acetoacetyl-coenzyme A synthesis and thiolysis Enterococcus faecalis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
(S)-3-hydroxy-3-methylglutaryl-CoA reduction Enterococcus faecalis
7
-
(R)-mevalonate oxidation Enterococcus faecalis
9.5
-
acetoacetyl-coenzyme A thiolysis Enterococcus faecalis
10.5
-
acetoacetyl-coenzyme A synthesis Enterococcus faecalis