Cloned (Comment) | Organism |
---|---|
DNA and amino acid sequence determination and analysis, sequence comparisons, cloning and expression in Escherichiaa coli strain JM109. Confirmation that the SD gene sequence originates from Phialocephala podophylli strain PPE7 fungal gDNA and is not a product from amplification of Podophyllum peltatum gDNA plant contamination is achieved through PCR amplification of any contaminating rbcL plant gene sequences in the PPE7 fungal gDNA template samples | Phialocephala podophylli |
gene SDH_Pp7, DNA and amino acid sequence determination and analysis, sequence comparisons, cloning and expression in Escherichia coli strain JM109, compatible codon optimization for expression in the heterologous host Pichia pastoris | Podophyllum peltatum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-secoisolariciresinol + 2 NAD+ | Podophyllum peltatum | - |
(-)-matairesinol + 2 NADH + 2 H+ | - |
? | |
(-)-secoisolariciresinol + 2 NAD+ | Phialocephala podophylli | - |
(-)-matairesinol + 2 NADH + 2 H+ | - |
? | |
(-)-secoisolariciresinol + 2 NAD+ | Phialocephala podophylli PPE7 | - |
(-)-matairesinol + 2 NADH + 2 H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phialocephala podophylli | A0A0M5K823 | isolated from the rhizomes of Podophyllum peltatum | - |
Phialocephala podophylli PPE7 | A0A0M5K823 | isolated from the rhizomes of Podophyllum peltatum | - |
Podophyllum peltatum | A0A0M4J2R3 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(-)-secoisolariciresinol + 2 NAD+ | - |
Podophyllum peltatum | (-)-matairesinol + 2 NADH + 2 H+ | - |
? | |
(-)-secoisolariciresinol + 2 NAD+ | - |
Phialocephala podophylli | (-)-matairesinol + 2 NADH + 2 H+ | - |
? | |
(-)-secoisolariciresinol + 2 NAD+ | - |
Phialocephala podophylli PPE7 | (-)-matairesinol + 2 NADH + 2 H+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 32000, about, sequence calculation | Podophyllum peltatum |
More | the enzyme forms a homotetramer composed of an alpha/beta single domain structure with a dinucleotide-binding Rossmann fold for the binding of NAD+ and an active site with a highly conserved catalytic triad of amino acids, Ser153, Tyr167 and Lys171 | Podophyllum peltatum |
Synonyms | Comment | Organism |
---|---|---|
PpSD | - |
Podophyllum peltatum |
SDH_Pp7 | - |
Podophyllum peltatum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Podophyllum peltatum |
30 | - |
assay at | Phialocephala podophylli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
assay at | Podophyllum peltatum |
8.8 | - |
assay at | Phialocephala podophylli |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Podophyllum peltatum | |
NAD+ | - |
Phialocephala podophylli |
General Information | Comment | Organism |
---|---|---|
metabolism | the enzyme is part of the podophyllotoxin, PPT, biosynthetic pathway | Podophyllum peltatum |
metabolism | the enzyme is part of the podophyllotoxin, PPT, biosynthetic pathway | Phialocephala podophylli |
additional information | the enzyme forms a homotetramer composed of an alpha/beta single domain structure with a dinucleotide-binding Rossmann fold for the binding of NAD+ and an active site with a highly conserved catalytic triad of amino acids, Ser153, Tyr167 and Lys171 | Podophyllum peltatum |
physiological function | the enzyme secoisolariciresinol dehydrogenase facilitates the dehydrogenation of secoisolariciresinol to form matairesinol, a mid-pathway intermediate product in podophyllotoxin, PPT, biosynthesis | Podophyllum peltatum |
physiological function | the enzyme secoisolariciresinol dehydrogenase facilitates the dehydrogenation of secoisolariciresinol to form matairesinol, a mid-pathway intermediate product in podophyllotoxin, PPT, biosynthesis | Phialocephala podophylli |