General Stability | Organism |
---|---|
stability of the purified enzyme disappears in sodium citrate buffer and potassium phosphate buffer for 24 h at 4°C, it increases continuously in Tris-HCl buffer and in glycine/NaOH buffer | Chlorobaculum tepidum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | N-acetylserotonin and melatonin are no inhibitors | Chlorobaculum tepidum | |
N-acetyldopamine | - |
Chlorobaculum tepidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0062 | - |
NADPH | pH 8.8, 45°C | Chlorobaculum tepidum | |
0.021 | - |
sepiapterin | pH 8.8, 45°C | Chlorobaculum tepidum | |
4 | - |
diacetyl | pH 8.8, 45°C | Chlorobaculum tepidum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | - |
Chlorobaculum tepidum | 5829 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
26000 | - |
2 * 26000, SDS-PAGE | Chlorobaculum tepidum |
55000 | - |
gel filtration | Chlorobaculum tepidum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH + H+ | Chlorobaculum tepidum | - |
L-threo-7,8-dihydrobiopterin + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Chlorobaculum tepidum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native enzyme 533fold from cytosol by ammonium sulfate fractionation, anion exchange chromatography, and gel filtration followed by affinity chromatography, another different step of anion exchange chromatography, and ultrafiltration | Chlorobaculum tepidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
6-pyruvoyl-5,6,7,8-tetrahydropterin + NADPH + H+ | - |
Chlorobaculum tepidum | L-threo-7,8-dihydrobiopterin + NADP+ | - |
? | |
diacetyl + NADPH + H+ | - |
Chlorobaculum tepidum | ? + NADP+ | - |
? | |
L-threo-7,8-dihydrobiopterin + NADP+ | - |
Chlorobaculum tepidum | sepiapterin + NADPH + H+ | - |
r | |
additional information | the enzyme also shows activity with D-threo-dihydrobiopterin, L-threo-dihydroneopterin, and dihydrotepidopterin, and low activity with L-erythro-dihydrobiopterin | Chlorobaculum tepidum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 26000, SDS-PAGE | Chlorobaculum tepidum |
Synonyms | Comment | Organism |
---|---|---|
sepiapterin reductase | - |
Chlorobaculum tepidum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
- |
Chlorobaculum tepidum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
5 | - |
NADPH | pH 8.8, 45°C | Chlorobaculum tepidum | |
5 | - |
sepiapterin | pH 8.8, 45°C | Chlorobaculum tepidum |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.8 | - |
- |
Chlorobaculum tepidum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NADPH | - |
Chlorobaculum tepidum |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.4 | - |
N-acetyldopamine | pH 8.8, 45°C | Chlorobaculum tepidum |
General Information | Comment | Organism |
---|---|---|
additional information | alignment of amino acid sequences near the N-termini of sepiapterin reductases from various sources, overview | Chlorobaculum tepidum |