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Literature summary for 1.1.1.318 extracted from

  • Singh, P.; Kalunke, R.M.; Shukla, A.; Tzfadia, O.; Thulasiram, H.V.; Giri, A.P.
    Biosynthesis and tissue-specific partitioning of camphor and eugenol in Ocimum kilimandscharicum (2020), Phytochemistry, 177, 112451 .
    View publication on PubMed

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
coniferyl acetate + NADPH + H+ Ocimum kilimandscharicum
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eugenol + acetate + NADP+
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r

Organism

Organism UniProt Comment Textmining
Ocimum kilimandscharicum A0A1B2U6T4
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-

Source Tissue

Source Tissue Comment Organism Textmining
flower
-
Ocimum kilimandscharicum
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inflorescence
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Ocimum kilimandscharicum
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leaf young and mature, gene egs1 is expressed in young leaves Ocimum kilimandscharicum
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additional information unlike camphor, eugenol accumulates more in roots. But the absence of the eugenol synthase gene in roots indicates long distance transport from aerial tissues. In silico co-expression analysis indicates the potential involvement of ATP-binding cassette, multidrug and toxic compound extrusion, and sugar transporters in eugenol transport. Ocimum kilimandscharicum roots accumulate a large amount of eugenol (56.6%) with low amount of camphor (16%). Gene egs1 is expressed in young leaves and flowers Ocimum kilimandscharicum
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
coniferyl acetate + NADPH + H+
-
Ocimum kilimandscharicum eugenol + acetate + NADP+
-
r

Synonyms

Synonyms Comment Organism
EGS1
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Ocimum kilimandscharicum
eugenol synthase 1
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Ocimum kilimandscharicum

Cofactor

Cofactor Comment Organism Structure
NADP+
-
Ocimum kilimandscharicum
NADPH
-
Ocimum kilimandscharicum

General Information

General Information Comment Organism
metabolism unlike camphor, eugenol accumulates more in roots. But the absence of the eugenol synthase gene in roots indicates long distance transport from aerial tissues. In silico co-expression analysis indicates the potential involvement of ATP-binding cassette, multidrug and toxic compound extrusion, and sugar transporters in eugenol transport. Ocimum spp. display metabolite partitioning between aerial and root tissues, strict partitioning of metabolites between the aerial shoot system (including young leaves, mature leaves, inflorescence, and flowers) and underground root system. Tissue-specific expression of terpenoid and phenylpropanoid pathway related genes in Ocimum kilimandscharicum. Transcriptomic analysis revealed the highest egs1 expression in young leaves followed by that in flowers, both of which accumulated considerably less amounts of eugenol. The sugar transporter(s) might be involved in partitioning eugenol in roots Ocimum kilimandscharicum