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Literature summary for 1.1.1.318 extracted from
- Characterization of a sweet basil acyltransferase involved in eugenol biosynthesis (2020), J. Exp. Bot., 71, 3638-3652 .
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene EGS1, recombinant expression of GST-tagged enzyme in Escherichia coli strain BL21(DE3). Transient coexpression of ObCAAT1 and/o ObCAAT2 with ObEGS1 under control of CMV 35S promoter in Nicotiana benthamiana leaves via Agrobacterium tumefaciens transfection, the transgenic leaves produce eugenol only when external coniferyl alcohol is added | Ocimum basilicum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferyl acetate + NADPH + H+ | Ocimum basilicum | - |
eugenol + acetate + NADP+ | - |
r |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ocimum basilicum | Q15GI4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-tagged enzyme EGS1 from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography | Ocimum basilicum |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | in leaves, not in leaf stripped of PGTs | Ocimum basilicum | - |
| additional information | no expression of EGS1 in roots | Ocimum basilicum | - |
| peltate glandular trichome | PGTs | Ocimum basilicum | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| coniferyl acetate + NADPH + H+ | - |
Ocimum basilicum | eugenol + acetate + NADP+ | - |
r | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 60000, recombinant GST-tagged enzyme, SDS-PAGE | Ocimum basilicum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| EGS1 | - |
Ocimum basilicum |
| eugenol synthase 1 | - |
Ocimum basilicum |
| ObEGS1 | - |
Ocimum basilicum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at room temperature | Ocimum basilicum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
assay at | Ocimum basilicum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| NADP+ | - |
Ocimum basilicum | |
| NADPH | - |
Ocimum basilicum | |
General Information
| General Information | Comment | Organism |
|---|---|---|
| metabolism | PGT-enriched BAHD acyltransferases, encoded by genes ObCAAT1 and ObCAAT2, convert coniferyl alcohol to coniferyl acetate which is the first committed step towards eugenol synthesis, followed by reduction of coniferyl acetate to eugenol and acetate catalyzed by eugenol synthase EGS1. Both ObCAAT1 and ObCAAT2 in combination with ObEGS1 produce eugenol The phenylpropene biosynthesis is not coupled to lignification in sweet basil. Plant phenylpropanoid pathway, overview | Ocimum basilicum |
| additional information | ObCAAT1-RNAi transgenic lines show decreased levels of eugenol and accumulation of coniferyl alcohol and its derivatives | Ocimum basilicum |
| physiological function | sweet basil (Ocimum basilicum) plants produce its characteristic phenylpropene-rich essential oil in specialized structures known as peltate glandular trichomes (PGTs). Eugenol and chavicol are the major phenylpropenes produced by sweet basil varieties. Eugenol is derived from coniferyl acetate by a reaction catalysed by eugenol synthase | Ocimum basilicum |
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