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Literature summary for 1.1.1.307 extracted from

  • Mayr, P.; Brueggler, K.; Kulbe, K.D.; Nidetzky, B.
    D-Xylose metabolism by Candida intermedia isolation and characterisation of two forms of aldose reductase with different coenzyme specificities (2000), J. Chromatogr. B Biomed. Sci. Appl., 737, 195-202 .
    View publication on PubMed

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.028
-
NADH pH 7, 25°C [Candida] intermedia
0.056
-
NADPH pH 7, 25°C [Candida] intermedia
50
-
D-xylose cosubstrate NADH, pH 7, 25°C [Candida] intermedia
52
-
D-xylose cosubstrate NADPH, pH 7, 25°C [Candida] intermedia

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
58000
-
gel filtration [Candida] intermedia

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
D-xylose + NADH + H+ [Candida] intermedia
-
xylitol + NAD+
-
?
D-xylose + NADPH + H+ [Candida] intermedia
-
xylitol + NADP+
-
?

Organism

Organism UniProt Comment Textmining
[Candida] intermedia
-
-
-

Purification (Commentary)

Purification (Comment) Organism
yeast cell extract is fractionated by biomimetic chromatography using the dye HE-3B (reactive Red 120) as pseudoaffinity ligand coupled to Sepharose CL-4B. In the second step, high-resolution anion-exchange chromatography using Mono Q yields purified isoform ALR2 in overall yieldof 62% [Candida] intermedia

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
D-xylose + NADH + H+
-
[Candida] intermedia xylitol + NAD+
-
?
D-xylose + NADPH + H+
-
[Candida] intermedia xylitol + NADP+
-
?

Subunits

Subunits Comment Organism
dimer 2 * 36000, SDS-PAGE, 2 * 35332, MALDI-TOF [Candida] intermedia

Synonyms

Synonyms Comment Organism
ALR2
-
[Candida] intermedia

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
9.8
-
D-xylose cosubstrate NADPH, pH 7, 25°C [Candida] intermedia
10.5
-
D-xylose cosubstrate NADH, pH 7, 25°C [Candida] intermedia
11
-
NADPH pH 7, 25°C [Candida] intermedia
11.2
-
NADH pH 7, 25°C [Candida] intermedia

pI Value

Organism Comment pI Value Maximum pI Value
[Candida] intermedia isoelectric focusing
-
4.6

General Information

General Information Comment Organism
physiological function isoform ALR1 is strictly specific for NADPH, EC 1.1.1.431, whereas isoform ALR2 utilises NADH and NADPH with similar specificity constants, EC 1.1.1.307 [Candida] intermedia

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.19
-
D-xylose cosubstrate NADPH, pH 7, 25°C [Candida] intermedia
0.21
-
D-xylose cosubstrate NADH, pH 7, 25°C [Candida] intermedia
200
-
NADPH pH 7, 25°C [Candida] intermedia
410
-
NADH pH 7, 25°C [Candida] intermedia