KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.028 | - |
NADH | pH 7, 25°C | [Candida] intermedia | |
0.056 | - |
NADPH | pH 7, 25°C | [Candida] intermedia | |
50 | - |
D-xylose | cosubstrate NADH, pH 7, 25°C | [Candida] intermedia | |
52 | - |
D-xylose | cosubstrate NADPH, pH 7, 25°C | [Candida] intermedia |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
58000 | - |
gel filtration | [Candida] intermedia |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADH + H+ | [Candida] intermedia | - |
xylitol + NAD+ | - |
? | |
D-xylose + NADPH + H+ | [Candida] intermedia | - |
xylitol + NADP+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
[Candida] intermedia | - |
- |
- |
Purification (Comment) | Organism |
---|---|
yeast cell extract is fractionated by biomimetic chromatography using the dye HE-3B (reactive Red 120) as pseudoaffinity ligand coupled to Sepharose CL-4B. In the second step, high-resolution anion-exchange chromatography using Mono Q yields purified isoform ALR2 in overall yieldof 62% | [Candida] intermedia |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
D-xylose + NADH + H+ | - |
[Candida] intermedia | xylitol + NAD+ | - |
? | |
D-xylose + NADPH + H+ | - |
[Candida] intermedia | xylitol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 36000, SDS-PAGE, 2 * 35332, MALDI-TOF | [Candida] intermedia |
Synonyms | Comment | Organism |
---|---|---|
ALR2 | - |
[Candida] intermedia |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
9.8 | - |
D-xylose | cosubstrate NADPH, pH 7, 25°C | [Candida] intermedia | |
10.5 | - |
D-xylose | cosubstrate NADH, pH 7, 25°C | [Candida] intermedia | |
11 | - |
NADPH | pH 7, 25°C | [Candida] intermedia | |
11.2 | - |
NADH | pH 7, 25°C | [Candida] intermedia |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
[Candida] intermedia | isoelectric focusing | - |
4.6 |
General Information | Comment | Organism |
---|---|---|
physiological function | isoform ALR1 is strictly specific for NADPH, EC 1.1.1.431, whereas isoform ALR2 utilises NADH and NADPH with similar specificity constants, EC 1.1.1.307 | [Candida] intermedia |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
D-xylose | cosubstrate NADPH, pH 7, 25°C | [Candida] intermedia | |
0.21 | - |
D-xylose | cosubstrate NADH, pH 7, 25°C | [Candida] intermedia | |
200 | - |
NADPH | pH 7, 25°C | [Candida] intermedia | |
410 | - |
NADH | pH 7, 25°C | [Candida] intermedia |